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Cited 19 time in webofscience Cited 21 time in scopus
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Biocompatibility evaluation of bioprinted decellularized collagen sheet implanted in vivo cornea using swept-source optical coherence tomography SCIE SCOPUS

Title
Biocompatibility evaluation of bioprinted decellularized collagen sheet implanted in vivo cornea using swept-source optical coherence tomography
Authors
Park, J.Lee, K.-P.Kim, H.Park, S.Wijesinghe, R.E.Lee, J.Han, S.Lee, S.Kim, P.Cho, D.-W.Jang, J.Kim, H.Jeon, M.Kim, J.
Date Issued
2019-06
Publisher
WILEY-V C H VERLAG GMBH
Abstract
Corneal transplantation by full-thickness penetrating keratoplasty with human donor tissue is a widely accepted treatment for damaged or diseased corneas. Although corneal transplantation has a high success rate, a shortage of high-quality donor tissue is a considerable limitation. Therefore, bioengineered corneas could be an effective solution for this limitation, and a decellularized extracellular matrix comprises a promising scaffold for their fabrication. In this study, three-dimensional bioprinted decellularized collagen sheets were implanted into the stromal layer of the cornea of five rabbits. We performed in vivo noninvasive monitoring of the rabbit corneas using swept-source optical coherence tomography (OCT) after implanting the collagen sheets. Anterior segment OCT images and averaged amplitude-scans were acquired biweekly to monitor corneal thickness after implantation for 1 month. The averaged cornea thickness in the control images was 430.3 +/- 5.9 mu m, while the averaged thickness after corneal implantation was 598.5 +/- 11.8 mu m and 564.5 +/- 12.5 mu m at 2 and 4 weeks, respectively. The corneal thickness reduction of 34 mu m confirmed the biocompatibility through the image analysis of the depth-intensity profile base. Moreover, hematoxylin and eosin staining supported the biocompatibility evaluation of the bioprinted decellularized collagen sheet implantation. Hence, the developed bioprinted decellularized collagen sheets could become an alternative solution to human corneal donor tissue, and the proposed image analysis procedure could be beneficial to confirm the success of the surgery.
URI
https://oasis.postech.ac.kr/handle/2014.oak/100268
DOI
10.1002/jbio.201900098
ISSN
1864-063X
Article Type
Article
Citation
JOURNAL OF BIOPHOTONICS, 2019-06
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조동우CHO, DONG WOO
Dept of Mechanical Enginrg
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