DC Field | Value | Language |
---|---|---|
dc.contributor.author | LEE, Jae-Jung | - |
dc.contributor.author | Kim, Jun-Young | - |
dc.contributor.author | Zhai, Duanting | - |
dc.contributor.author | Yun, Seong-Wook | - |
dc.contributor.author | Chang, Young-Tae | - |
dc.date.accessioned | 2019-12-18T06:30:03Z | - |
dc.date.available | 2019-12-18T06:30:03Z | - |
dc.date.created | 2019-12-18 | - |
dc.date.issued | 2012-06 | - |
dc.identifier.issn | 2005-8543 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/100521 | - |
dc.description.abstract | Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Westernblotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose “Estern staining”, as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming “immuno-staining” in many versatile applications. | - |
dc.language | English | - |
dc.publisher | KISTI | - |
dc.relation.isPartOf | Interdisciplinary Bio Central | - |
dc.title | Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Counter Partner Which is a Fluorescent Compound | - |
dc.type | Article | - |
dc.identifier.doi | 10.4051 / ibc.2012.4.2.0005 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Interdisciplinary Bio Central, v.4, no.5, pp.1 - 9 | - |
dc.citation.endPage | 9 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | Interdisciplinary Bio Central | - |
dc.citation.volume | 4 | - |
dc.contributor.affiliatedAuthor | Chang, Young-Tae | - |
dc.description.journalClass | 2 | - |
dc.description.journalClass | 2 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | other | - |
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