DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryu, CH | - |
dc.contributor.author | Park, SH | - |
dc.contributor.author | Park, SA | - |
dc.contributor.author | Kim, SM | - |
dc.contributor.author | Lim, JY | - |
dc.contributor.author | Jeong, CH | - |
dc.contributor.author | Yoon, WS | - |
dc.contributor.author | Oh, WI | - |
dc.contributor.author | Sung, YC | - |
dc.contributor.author | Jeun, SS | - |
dc.date.accessioned | 2015-06-25T01:57:12Z | - |
dc.date.available | 2015-06-25T01:57:12Z | - |
dc.date.created | 2011-07-11 | - |
dc.date.issued | 2011-06 | - |
dc.identifier.issn | 1043-0342 | - |
dc.identifier.other | 2015-OAK-0000023783 | en_US |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/10220 | - |
dc.description.abstract | Clinical trials of gene therapy using a viral delivery system for glioma have been limited. Recently, gene therapy using stem cells as the vehicles for delivery of therapeutic agents has emerged as a new treatment strategy for malignant brain tumors. In this study, we used human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) as delivery vehicles with glioma-targeting capabilities, and modified interleukin-12 (IL-12p40N220Q; IL-12M) as a novel therapeutic gene. We also engineered UCB-MSCs to secret IL-12M(UCB-MSC-IL12M) via tetrameric cell-permeable peptide (4HP4)-mediated adenoviral transduction. We confirmed the migratory capacity of UCB-MSC-IL12M toward GL26 mouse glioma cells by an in vitro migration assay and in vivo injection of UCB-MSC-IL12M into the ipsilateral hemisphere of implanted gliomas in C57BL/6 mice. In vivo efficacy experiments showed that intratumoral injection of UCB-MSC-IL12M significantly inhibited tumor growth and prolonged the survival of glioma-bearing mice compared with control mice. Antitumor effects were associated with increased local IL-12M levels, followed by interferon-g secretion and T-cell infiltration in intracranial gliomas, as well as antiangiogenesis. Interestingly, tumor-free mice after UCB-MSC-IL12M treatment were resistant to ipsilateral and contralateral tumor rechallenge, which was closely associated with tumor-specific long-term T-cell immunity. Thus, our results provide the rationale for designing novel experimental protocols to induce long-term antitumor immunity against intracranial gliomas using UCB-MSCs as an effective delivery vehicle for therapeutic cytokines including IL-12M. | - |
dc.description.statementofresponsibility | open | en_US |
dc.language | English | - |
dc.publisher | MARY ANN LIEBERT INC | - |
dc.relation.isPartOf | HUMAN GENE THERAPY | - |
dc.rights | BY_NC_ND | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.0/kr | en_US |
dc.title | Gene Therapy of Intracranial Glioma Using Interleukin 12-Secreting Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | en_US |
dc.identifier.doi | 10.1089/HUM.2010.187 | - |
dc.author.google | Ryu, CH | en_US |
dc.author.google | Park, SH | en_US |
dc.author.google | Jeun, SS | en_US |
dc.author.google | Sung, YC | en_US |
dc.author.google | Oh, WI | en_US |
dc.author.google | Yoon, WS | en_US |
dc.author.google | Jeong, CH | en_US |
dc.author.google | Lim, JY | en_US |
dc.author.google | Kim, SM | en_US |
dc.author.google | Park, SA | en_US |
dc.relation.volume | 22 | en_US |
dc.relation.issue | 6 | en_US |
dc.relation.startpage | 733 | en_US |
dc.relation.lastpage | 743 | en_US |
dc.contributor.id | 10053752 | en_US |
dc.relation.journal | HUMAN GENE THERAPY | en_US |
dc.relation.index | SCI급, SCOPUS 등재논문 | en_US |
dc.relation.sci | SCI | en_US |
dc.collections.name | Journal Papers | en_US |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | HUMAN GENE THERAPY, v.22, no.6, pp.733 - 743 | - |
dc.identifier.wosid | 000291388300084 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 743 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 733 | - |
dc.citation.title | HUMAN GENE THERAPY | - |
dc.citation.volume | 22 | - |
dc.contributor.affiliatedAuthor | Sung, YC | - |
dc.identifier.scopusid | 2-s2.0-79955034826 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 71 | - |
dc.description.scptc | 79 | * |
dc.date.scptcdate | 2018-10-274 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CENTRAL-NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | RECOMBINANT INTERLEUKIN-12 | - |
dc.subject.keywordPlus | MALIGNANT GLIOMAS | - |
dc.subject.keywordPlus | TUMOR | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | IL-12 | - |
dc.subject.keywordPlus | RAT | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordPlus | CANCER | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
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