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Cited 8 time in webofscience Cited 8 time in scopus
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dc.contributor.authorKim, Sang-Jin-
dc.contributor.authorKim, Jiyoung-
dc.contributor.authorKim, Bonah-
dc.contributor.authorLee, Won-Woo-
dc.contributor.authorLiu, Xiao-
dc.contributor.authorChang, Young-Tae-
dc.contributor.authorPark, Jong-Wan-
dc.date.accessioned2020-07-14T00:50:38Z-
dc.date.available2020-07-14T00:50:38Z-
dc.date.created2020-06-23-
dc.date.issued2020-06-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://oasis.postech.ac.kr/handle/2014.oak/103867-
dc.description.abstractNeutrophil extracellular trap (NET) is one of the first-line defenses against microbes. Under certain circumstances, however, it also plays an aggravating factor in diverse inflammation-related diseases including cancers and vascular diseases. Our aim is to develop a new method to detect NET in cells and tissues using a DNA-specific fluorescence probe CDr15. CDr15 was characterized to be impermeable to the cell membranes and to emit a strong fluorescence in association with extracellular DNAs in NET. Due to these properties, CDr15 was successfully shown to quantify NETs in vitro and to be applicable for real-time monitoring NET formation in PMA-stimulated neutrophils. Even in formaldehyde-fixed tumor specimens, CDr15 could detect NETs spreading around cancer cells. Compared with DAPI and SYTOX DNA dyes, CDr15 showed a lower level of background fluorescence and a higher specificity in NET detection. Based on these results, we propose CDr15 as a novel marker of NET to be applicable in experimental and clinical studies. (C) 2020 Elsevier Inc. All rights reserved.-
dc.languageEnglish-
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.titleValidation of CDr15 as a new dye for detecting neutrophil extracellular trap-
dc.typeArticle-
dc.identifier.doi10.1016/j.bbrc.2020.04.153-
dc.type.rimsART-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.527, no.3, pp.646 - 653-
dc.identifier.wosid000537622800009-
dc.citation.endPage653-
dc.citation.number3-
dc.citation.startPage646-
dc.citation.titleBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.citation.volume527-
dc.contributor.affiliatedAuthorLiu, Xiao-
dc.contributor.affiliatedAuthorChang, Young-Tae-
dc.identifier.scopusid2-s2.0-85084641810-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.type.docTypeArticle-
dc.subject.keywordPlusNETOSIS-
dc.subject.keywordPlusPROGRESSION-
dc.subject.keywordPlusPROMOTE-
dc.subject.keywordPlusNETS-
dc.subject.keywordPlusDNA-
dc.subject.keywordAuthorNeutrophil extracellular trap-
dc.subject.keywordAuthorCDr15-
dc.subject.keywordAuthorDNA dye-
dc.subject.keywordAuthorFluorescence imaging-
dc.relation.journalWebOfScienceCategoryBiochemistry & Molecular Biology-
dc.relation.journalWebOfScienceCategoryBiophysics-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.relation.journalResearchAreaBiophysics-

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