DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sang-Jin | - |
dc.contributor.author | Kim, Jiyoung | - |
dc.contributor.author | Kim, Bonah | - |
dc.contributor.author | Lee, Won-Woo | - |
dc.contributor.author | Liu, Xiao | - |
dc.contributor.author | Chang, Young-Tae | - |
dc.contributor.author | Park, Jong-Wan | - |
dc.date.accessioned | 2020-07-14T00:50:38Z | - |
dc.date.available | 2020-07-14T00:50:38Z | - |
dc.date.created | 2020-06-23 | - |
dc.date.issued | 2020-06 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/103867 | - |
dc.description.abstract | Neutrophil extracellular trap (NET) is one of the first-line defenses against microbes. Under certain circumstances, however, it also plays an aggravating factor in diverse inflammation-related diseases including cancers and vascular diseases. Our aim is to develop a new method to detect NET in cells and tissues using a DNA-specific fluorescence probe CDr15. CDr15 was characterized to be impermeable to the cell membranes and to emit a strong fluorescence in association with extracellular DNAs in NET. Due to these properties, CDr15 was successfully shown to quantify NETs in vitro and to be applicable for real-time monitoring NET formation in PMA-stimulated neutrophils. Even in formaldehyde-fixed tumor specimens, CDr15 could detect NETs spreading around cancer cells. Compared with DAPI and SYTOX DNA dyes, CDr15 showed a lower level of background fluorescence and a higher specificity in NET detection. Based on these results, we propose CDr15 as a novel marker of NET to be applicable in experimental and clinical studies. (C) 2020 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.title | Validation of CDr15 as a new dye for detecting neutrophil extracellular trap | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2020.04.153 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.527, no.3, pp.646 - 653 | - |
dc.identifier.wosid | 000537622800009 | - |
dc.citation.endPage | 653 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 646 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 527 | - |
dc.contributor.affiliatedAuthor | Liu, Xiao | - |
dc.contributor.affiliatedAuthor | Chang, Young-Tae | - |
dc.identifier.scopusid | 2-s2.0-85084641810 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | NETOSIS | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordPlus | PROMOTE | - |
dc.subject.keywordPlus | NETS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordAuthor | Neutrophil extracellular trap | - |
dc.subject.keywordAuthor | CDr15 | - |
dc.subject.keywordAuthor | DNA dye | - |
dc.subject.keywordAuthor | Fluorescence imaging | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
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