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Cited 13 time in webofscience Cited 14 time in scopus
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iNrich, Rapid and Robust Method to Enrich N-Terminal Proteome in a Highly Multiplexed Platform SCIE SCOPUS

Title
iNrich, Rapid and Robust Method to Enrich N-Terminal Proteome in a Highly Multiplexed Platform
Authors
Ju, ShinyeongKwon, YumiKim, Jeong-MokPark, DaechanLee, SeonjeongLee, Jin-WonHwang, Cheol-SangLee, Cheolju
Date Issued
2020-05
Publisher
AMER CHEMICAL SOC
Abstract
The field of terminal proteomics is limited in that it is optimized for large-scale analysis via multistep processes involving liquid chromatography. Here, we present an integrated N-terminal peptide enrichment method (iNrich) that can handle as little as 25 mu g of cell lysate via a single-stage encapsulated solid-phase extraction column. iNrich enables simple, rapid, and reproducible sample processing, treatment of a wide range of protein amounts (25 mu g similar to 1 mg), multiplexed parallel sample preparation, and in-stage sample prefractionation using a mixed-anion-exchange filter. We identified similar to 5000 N-terminal peptides (Nt-peptides) from only 100 mu g of human cell lysate including Nt-formyl peptides. Multiplexed sample preparation facilitated quantitative and robust enrichment of N-terminome iNrich with dozens of samples simultaneously. We further developed the method to incorporate isobaric tags such as a tandem mass tag (TMT) and used it to discover novel peptides during ER stress analysis. The iNrich facilitated high-throughput N-terminomics and degradomics at a low cost using commercially available reagents and apparatus, without requiring arduous procedures.
URI
https://oasis.postech.ac.kr/handle/2014.oak/104830
DOI
10.1021/acs.analchem.9b05653
ISSN
0003-2700
Article Type
Article
Citation
ANALYTICAL CHEMISTRY, vol. 92, no. 9, page. 6462 - 6469, 2020-05
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황철상HWANG, CHEOL SANG
Dept of Life Sciences
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