DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, J. | - |
dc.contributor.author | Nan, N. | - |
dc.contributor.author | Li, N. | - |
dc.contributor.author | Liu, Y. | - |
dc.contributor.author | Wang, T.-J. | - |
dc.contributor.author | Hwang, I. | - |
dc.contributor.author | Liu, B. | - |
dc.contributor.author | Xu, Z.-Y. | - |
dc.date.accessioned | 2021-06-01T04:07:57Z | - |
dc.date.available | 2021-06-01T04:07:57Z | - |
dc.date.created | 2020-11-26 | - |
dc.date.issued | 2020-11 | - |
dc.identifier.issn | 1040-4651 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/105424 | - |
dc.description.abstract | OsSUVH7, OsBAG4, and OsMYB106 form a DNA methylation reader-chaperone regulator-transcription factor complex on the promoter region of OsHKT1;5 to activate its expression during salinity stress. Irrigated lands are increasingly salinized, which adversely affects agricultural productivity. To respond to high sodium (Na+) concentrations, plants harbor multiple Na+ transport systems. Rice (Oryza sativa) HIGH-AFFINITY POTASSIUM (K+) TRANSPORTER1;5 (OsHKT1;5), a Na+-selective transporter, maintains K+/Na+ homeostasis under salt stress. However, the mechanism regulating OsHKT1;5 expression remains unknown. Here, we present evidence that a protein complex consisting of rice BCL-2-ASSOCIATED ATHANOGENE4 (OsBAG4), OsMYB106, and OsSUVH7 regulates OsHKT1;5 expression in response to salt stress. We isolated a salt stress-sensitive mutant, osbag4-1, that showed significantly reduced OsHKT1;5 expression and reduced K+ and elevated Na+ levels in shoots. Using comparative interactomics, we isolated two OsBAG4-interacting proteins, OsMYB106 (a MYB transcription factor) and OsSUVH7 (a DNA methylation reader), that were crucial for OsHKT1;5 expression. OsMYB106 and OsSUVH7 bound to the MYB binding cis-element (MYBE) and the miniature inverted-repeat transposable element (MITE) upstream of the MYBE, respectively, in the OsHKT1;5 promoter. OsBAG4 functioned as a bridge between OsSUVH7 and OsMYB106 to facilitate OsMYB106 binding to the consensus MYBE in the OsHKT1;5 promoter, thereby activating the OsHKT1;5 expression. Elimination of the MITE or knockout of OsMYB106 or OsSUVH7 decreased OsHKT1;5 expression and increased salt sensitivity. Our findings reveal a transcriptional complex, consisting of a DNA methylation reader, a chaperone regulator, and a transcription factor, that collaboratively regulate OsHKT1;5 expression during salinity stress. | - |
dc.language | English | - |
dc.publisher | AMER SOC PLANT BIOLOGISTS | - |
dc.relation.isPartOf | PLANT CELL | - |
dc.title | A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress | - |
dc.type | Article | - |
dc.identifier.doi | 10.1105/tpc.20.00301 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PLANT CELL, v.32, no.11, pp.3535 - 3558 | - |
dc.identifier.wosid | 000600226800018 | - |
dc.citation.endPage | 3558 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 3535 | - |
dc.citation.title | PLANT CELL | - |
dc.citation.volume | 32 | - |
dc.contributor.affiliatedAuthor | Hwang, I. | - |
dc.identifier.scopusid | 2-s2.0-85095460871 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RESPONSIVE GENE-EXPRESSION | - |
dc.subject.keywordPlus | SALT TOLERANCE | - |
dc.subject.keywordPlus | BCL-2-BINDING PROTEIN | - |
dc.subject.keywordPlus | FUNCTIONAL-ANALYSIS | - |
dc.subject.keywordPlus | HKT TRANSPORTERS | - |
dc.subject.keywordPlus | POTASSIUM UPTAKE | - |
dc.subject.keywordPlus | ARABIDOPSIS | - |
dc.subject.keywordPlus | NA+ | - |
dc.subject.keywordPlus | RICE | - |
dc.subject.keywordPlus | SODIUM | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Cell Biology | - |
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