DC Field | Value | Language |
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dc.contributor.author | Walton, Travis | - |
dc.contributor.author | DasGupta, Saurja | - |
dc.contributor.author | Duzdevich, Daniel | - |
dc.contributor.author | Ohc, Seung Soo | - |
dc.contributor.author | Szostak, Jack W. | - |
dc.date.accessioned | 2021-06-01T05:06:07Z | - |
dc.date.available | 2021-06-01T05:06:07Z | - |
dc.date.created | 2020-04-20 | - |
dc.date.issued | 2020-03 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/105621 | - |
dc.description.abstract | The hypothesized central role of RNA in the origin of life suggests that RNA propagation predated the advent of complex protein enzymes. A critical step of RNA replication is the template-directed synthesis of a complementary strand. Two experimental approaches have been extensively explored in the pursuit of demonstrating protein-free RNA synthesis: template-directed nonenzymatic RNA polymerization using intrinsically reactive monomers and ribozyme-catalyzed polymerization using more stable substrates such as biological 5'-triphosphates. Despite significant progress in both approaches in recent years, the assembly and copying of functional RNA sequences under prebiotic conditions remains a challenge. Here, we explore an alternative approach to RNA-templated RNA copying that combines ribozyme catalysis with RNA substrates activated with a prebiotically plausible leaving group, 2-aminoimidazole (2AI). We applied in vitro selection to identify ligase ribozymes that catalyze phosphodiester bond formation between a template-bound primer and a phosphor-imidazolide-activated oligomer. Sequencing revealed the progressive enrichment of 10 abundant sequences from a random sequence pool. Ligase activity was detected in all 10 RNA sequences; all required activation of the ligator with 2AI and generated a 3'-5' phosphodiester bond. We propose that ribozyme catalysis of phosphodiester bond formation using intrinsically reactive RNA substrates, such as imidazolides, could have been an evolutionary step connecting purely nonenzymatic to ribozyme-catalyzed RNA template copying during the origin of life. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.title | In vitro selection of ribozyme ligases that use prebiotically plausible 2-aminoimidazole-activated substrates | - |
dc.type | Article | - |
dc.identifier.doi | 10.1073/pnas.1914367117 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.117, no.11, pp.5741 - 5748 | - |
dc.identifier.wosid | 000520011000032 | - |
dc.citation.endPage | 5748 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 5741 | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 117 | - |
dc.contributor.affiliatedAuthor | Ohc, Seung Soo | - |
dc.identifier.scopusid | 2-s2.0-85081670087 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TEMPLATE-DIRECTED SYNTHESIS | - |
dc.subject.keywordPlus | PRIMER EXTENSION | - |
dc.subject.keywordPlus | RNA POLYMERIZATION | - |
dc.subject.keywordPlus | STRUCTURAL BASIS | - |
dc.subject.keywordPlus | LIGATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | ACCURATE | - |
dc.subject.keywordPlus | ORIGIN | - |
dc.subject.keywordPlus | TOOLS | - |
dc.subject.keywordAuthor | ribozymes | - |
dc.subject.keywordAuthor | ligation | - |
dc.subject.keywordAuthor | prebiotic | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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