Development of high-throughput drug screening platform that reproduces the gastric cancer-specific microenvironment using 3D Bioprinting technology

Abstract

In recent years, in vitro anticancer drug toxicity evaluation and drug screening tests have been increasingly attempted for the development of new drugs under conditions that reproduce an environment similar to the human body through 3D cell culture. Also, various studies have been conducted in the development of patient-derived xenograft (PDX) for investigating the efficacy of anti-cancer drugs. However, they still have the critical limitations, we have established in vitro gastric cancer PDX culture conditions using porcine gastric tissue-derived decellularized extracellular matrix (gastric dECM) to overcome their limitation. Our system provide a microenvironment for PDX growth in vitro. This process can improve production efficiency, reduce the time and cost for sub-culturing PDX and achieve mass production for drug screening. Furthermore, we confirmed different drug resistance depending on the type of PDX. These in vitro PDX culture platform might be used for various cancer drug screening as well as evaluation method to validate rapid patient-specific drug response.