Escherichia coli W as a new platform strain for the enhanced production of L-valine by systems metabolic engineering
SCIE
SCOPUS
- Title
- Escherichia coli W as a new platform strain for the enhanced production of L-valine by systems metabolic engineering
- Authors
- Park, Jin Hwan; Jang, Yu-Sin; Lee, Jeong Wook; Lee, Sang Yup
- Date Issued
- 2011-05
- Publisher
- Wiley - V C H Verlag GmbbH & Co.
- Abstract
- A less frequently employed Escherichia coli strain W, yet possessing useful metabolic characteristics such as less acetic acid production and high L-valine tolerance, was metabolically engineered for the production of L-valine. The ilvA gene was deleted to make more pyruvate, a key precursor for L-valine, available for enhanced L-valine biosynthesis. The lacI gene was deleted to allow constitutive expression of genes under the tac or trc promoter. The ilvBN(mut) genes encoding feedback-resistant acetohydroxy acid synthase (AHAS) I and the L-valine biosynthetic ilvCED genes encoding acetohydroxy acid isomeroreductase, dihydroxy acid dehydratase, and branched chain amino acid aminotransferase, respectively, were amplified by plasmid-based overexpression. The global regulator Lrp and L-valine exporter YgaZH were also amplified by plasmid-based overexpression. The engineered E. coli W (Delta lacI Delta ilvA) strain overexpressing the ilvBN(mut), ilvCED, ygaZH, and lrp genes was able to produce an impressively high concentration of 60.7 g/L L-valine by fed-batch culture in 29.5 h, resulting in a high volumetric productivity of 2.06 g/L/h. The most notable finding is that there was no other byproduct produced during L-valine production. The results obtained in this study suggest that E. coli W can be a good alternative to Corynebacterium glutamicum and E. coli K-12, which have so far been the most efficient L-valine producer. Furthermore, it is expected that various bioproducts including other amino acids might be more efficiently produced by this revisited platform strain of E. coli. Biotechnol. Bioeng. 2011;108: 1140-1147. (C) 2010 Wiley Periodicals, Inc.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/105763
- DOI
- 10.1002/bit.23044
- ISSN
- 0006-3592
- Article Type
- Article
- Citation
- Biotechnology and Bioengineering, vol. 108, no. 5, page. 1140 - 1147, 2011-05
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.