Baleen-Mimicking Virtual Filters for Rapid Detection of Pathogenic Bacteria in Water Using Magnetic Nanoparticle Chains and a Halbach Ring
SCIE
SCOPUS
- Title
- Baleen-Mimicking Virtual Filters for Rapid Detection of Pathogenic Bacteria in Water Using Magnetic Nanoparticle Chains and a Halbach Ring
- Authors
- Lee, Hyeonjeong; Han, Hyunsoo; Jeon, Sangmin
- Date Issued
- 2020-11
- Publisher
- AMER CHEMICAL SOC
- Abstract
- We have developed a virtual filter that quickly and efficiently captures and detects pathogenic bacteria in large amounts of water. The virtual filter comprised magnetic nanoparticle chains (MNCs) obtained by cross-linking alginate-coated magnetic nanoparticles (MNPs). When the MNC solution in a disposable plastic tube was exposed to an external magnetic field, the MNCs were aligned along the magnetic field lines, forming a filter similar to a whale's baleen filtering system. A Halbach ring that increased the magnetic field inside the ring was used as the source of an external magnetic field. The Halbach ring produced a more uniform and denser alignment of MNCs than any other ring array. To demonstrate the performance of the virtual filter comprising MNCs, Escherichia coli (E. coli) O157-spiked water was injected into the virtual filter comprising polyethyleneimine-coated MNCs, and the concentration of E. coli O157 captured by electrostatic interaction was determined using qPCR analysis. The bacterial capture efficiency using MNCs was 90% at a flow rate of 5 mL/min, and the detection limit after 10 min of preconcentration of bacteria was 10 cfu/mL, which is 100 times lower than that obtained using qPCR alone.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/106659
- DOI
- 10.1021/acssensors.0c01334
- ISSN
- 2379-3694
- Article Type
- Article
- Citation
- ACS SENSORS, vol. 5, no. 11, page. 3432 - 3437, 2020-11
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- There are no files associated with this item.
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