DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Dongsic | - |
dc.contributor.author | Go, Gyeongyun | - |
dc.contributor.author | Kim, Dae-Kyum | - |
dc.contributor.author | Lee, Jaewook | - |
dc.contributor.author | Park, Seon-Min | - |
dc.contributor.author | Di Vizio, Dolores | - |
dc.contributor.author | Gho, Yong Song | - |
dc.date.accessioned | 2021-09-03T04:23:34Z | - |
dc.date.available | 2021-09-03T04:23:34Z | - |
dc.date.created | 2020-05-21 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.issn | 2001-3078 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/106964 | - |
dc.description.abstract | Extracellular vesicles (EVs) are nano-sized vesicles surrounded by a lipid bilayer and released into the extracellular milieu by most of cells. Although various EV isolation methods have been established, most of the current methods isolate EVs with contaminated non-vesicular proteins. By applying the label-free quantitative proteomic analyses of human colon cancer cell SW480-derived EVs, we identified trypsin-sensitive and trypsin-resistant vesicular proteins. Further systems biology and protein-protein interaction network analyses based on their cellular localization, we classified the trypsin-sensitive and trypsin-resistant vesicular proteins into two subgroups: 363 candidate real-vesicular proteins and 151 contaminated non-vesicular proteins. Moreover, the protein interaction network analyses showed that candidate real-vesicular proteins are mainly derived from plasma membrane (46.8%), cytosol (36.6%), cytoskeleton (8.0%) and extracellular region (2.5%). On the other hand, most of the contaminated non-vesicular proteins derived from nucleus, Golgi apparatus, endoplasmic reticulum and mitochondria. In addition, ribosomal protein complexes and T-complex proteins were classified as the contaminated non-vesicular proteins. Taken together, our trypsin-digested proteomic approach on EVs is an important advance to identify the real-vesicular proteins that could help to understand EV biogenesis and protein cargo-sorting mechanism during EV release, to identify more reliable EV diagnostic marker proteins, and to decode pathophysiological roles of EVs. | - |
dc.language | English | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.relation.isPartOf | JOURNAL OF EXTRACELLULAR VESICLES | - |
dc.title | Quantitative proteomic analysis of trypsin-treated extracellular vesicles to identify the real-vesicular proteins | - |
dc.type | Article | - |
dc.identifier.doi | 10.1080/20013078.2020.1757209 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF EXTRACELLULAR VESICLES, v.9, no.1 | - |
dc.identifier.wosid | 000531002300001 | - |
dc.citation.number | 1 | - |
dc.citation.title | JOURNAL OF EXTRACELLULAR VESICLES | - |
dc.citation.volume | 9 | - |
dc.contributor.affiliatedAuthor | Go, Gyeongyun | - |
dc.contributor.affiliatedAuthor | Gho, Yong Song | - |
dc.identifier.scopusid | 2-s2.0-85084368847 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | EXOSOMES | - |
dc.subject.keywordPlus | TRANSCRIPTOMICS | - |
dc.subject.keywordPlus | MICROVESICLES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | DATABASE | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | EVPEDIA | - |
dc.subject.keywordAuthor | Extracellular vesicles | - |
dc.subject.keywordAuthor | exosomesproteomics | - |
dc.subject.keywordAuthor | trypsin | - |
dc.subject.keywordAuthor | protease | - |
dc.subject.keywordAuthor | contaminated non-vesicular proteins | - |
dc.subject.keywordAuthor | ultracentrifuge | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
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