DC Field | Value | Language |
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dc.contributor.author | CHOI, KYUHA | - |
dc.date.accessioned | 2021-09-03T04:32:00Z | - |
dc.date.available | 2021-09-03T04:32:00Z | - |
dc.date.created | 2021-08-17 | - |
dc.date.issued | 2021-08-13 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/106971 | - |
dc.description.abstract | Meiosis is critical to sexual reproduction and creates genetic diversity in most eukaryotes. During meiosis, homologous chromosomes pair and undergo reciprocal genetic exchange, called crossover. Crossovers have a profound effect on genetic diversity, and are a critical process during crop improvement, allowing new recombinant backgrounds to be generated. Meiotic crossover number is tightly restricted along chromosomes in most eukaryotes, despite an excess of initiating DNA double-strand breaks. The majority of plant crossovers are dependent on class I interfering repair pathway, with a minority formed via the class II pathway. Class II crossover is limited by anti-recombination pathways; however, similar pathways repressing class I crossovers have not been identified. We performed a forward genetic screen in Arabidopsis using fluorescent crossover reporters to identify high crossover rate (hcr) mutants. We identified HCR1, HCR2 and HCR3 as repressing class I crossovers. Genetic proof, cytological results, genome wide crossover mapping and high-throughput fluorescence reporter analyses revealed that HCR1 and HCR3 limit crossovers by controlling protein phosphorylation and interactions of pro-crossover factors in class I pathway, while HCR2 represses transcription of HEI10 encoding meiotic E3 ligase that promotes crossover number in a dosage dependent manner. Our findings shed light on new anti-recombination pathways by which class I interfering crossovers are restricted. I will present our genetic screen, advanced approaches and ongoing studies on meiotic crossover recombination in plants. | - |
dc.language | Korean | - |
dc.publisher | 한국식물생명공학회 | - |
dc.relation.isPartOf | 2021년 한국식물생명공학회 국제학술발표회 및 총회 | - |
dc.title | 2021년 한국식물생명공학회 국제학술발표회 및 총회 | - |
dc.title.alternative | Genetic dissection of anti-crossover recombination pathways | - |
dc.type | Conference | - |
dc.type.rims | CONF | - |
dc.identifier.bibliographicCitation | 2021년 한국식물생명공학회 국제학술발표회 및 총회 | - |
dc.citation.conferenceDate | 2021-08-12 | - |
dc.citation.conferencePlace | KO | - |
dc.citation.conferencePlace | 제주도, 라마다 호텔 | - |
dc.citation.title | 2021년 한국식물생명공학회 국제학술발표회 및 총회 | - |
dc.contributor.affiliatedAuthor | CHOI, KYUHA | - |
dc.description.journalClass | 2 | - |
dc.description.journalClass | 2 | - |
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