DC Field | Value | Language |
---|---|---|
dc.contributor.author | LIM, GEUN BAE | - |
dc.contributor.author | Jongyoon Han | - |
dc.contributor.author | Hyunryul Ryu | - |
dc.contributor.author | Bruce D. Levy | - |
dc.contributor.author | Kyungyong Choi | - |
dc.contributor.author | Bakr Jundi | - |
dc.contributor.author | JEON, HYUNG KOOK | - |
dc.date.accessioned | 2021-09-24T05:50:41Z | - |
dc.date.available | 2021-09-24T05:50:41Z | - |
dc.date.created | 2020-09-21 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/107144 | - |
dc.description.abstract | A fully-automated and portable leukocyte separation platform was developed based on a new type of inertial microfluidic device, multi-dimensional double spiral (MDDS) device, as an alternative to centrifugation. By combining key innovations in inertial microfluidic device designs and check-valve-based recirculation processes, highly purified and concentrated WBCs (up to >99.99% RBC removal, similar to 80% WBC recovery, >85% WBC purity, and similar to 12-fold concentrated WBCs compared to the input sample) were achieved in less than 5 minutes, with high reliability and repeatability (coefficient of variation, CV < 5%). Using this, one can harvest up to 0.4 million of intact WBCs from 50 mu L of human peripheral blood (50 mu L), without any cell damage or phenotypic changes in a fully-automated operation. Alternatively, hand-powered operation is demonstrated with comparable separation efficiency and speed, which eliminates the need for electricity altogether for truly field-friendly sample preparation. The proposed platform is therefore highly deployable for various point-of-care applications, including bedside assessment of the host immune response and blood sample processing in resource-limited environments. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.relation.isPartOf | LAB ON A CHIP | - |
dc.title | Fully-automated and field-deployable blood leukocyte separation platform using multi-dimensional double spiral (MDDS) inertial microfluidics | - |
dc.type | Article | - |
dc.identifier.doi | 10.1039/d0lc00675k | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | LAB ON A CHIP, v.20, no.19, pp.3612 - 3624 | - |
dc.identifier.wosid | 000573314400009 | - |
dc.citation.endPage | 3624 | - |
dc.citation.number | 19 | - |
dc.citation.startPage | 3612 | - |
dc.citation.title | LAB ON A CHIP | - |
dc.citation.volume | 20 | - |
dc.contributor.affiliatedAuthor | LIM, GEUN BAE | - |
dc.contributor.affiliatedAuthor | JEON, HYUNG KOOK | - |
dc.identifier.scopusid | 2-s2.0-85092249048 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | LABEL-FREE ISOLATION | - |
dc.subject.keywordPlus | HIGH-THROUGHPUT SEPARATION | - |
dc.subject.keywordPlus | CIRCULATING TUMOR-CELLS | - |
dc.subject.keywordPlus | ULTRA-FAST | - |
dc.subject.keywordPlus | WHOLE-BLOOD | - |
dc.subject.keywordPlus | REAL-TIME | - |
dc.subject.keywordPlus | RT-PCR | - |
dc.subject.keywordPlus | MICROCHANNELS | - |
dc.subject.keywordPlus | CHANNEL | - |
dc.subject.keywordPlus | CANCER | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.