DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Ji Yeon | - |
dc.contributor.author | Cha, Sanghak | - |
dc.contributor.author | Lee, Ji Hoon | - |
dc.contributor.author | Lim, Hyun Gyu | - |
dc.contributor.author | Noh, Myung Hyun | - |
dc.contributor.author | Kang, Chae Won | - |
dc.contributor.author | Jung, Gyoo Yeol | - |
dc.date.accessioned | 2021-11-16T09:50:23Z | - |
dc.date.available | 2021-11-16T09:50:23Z | - |
dc.date.created | 2021-10-12 | - |
dc.date.issued | 2021-09 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/107485 | - |
dc.description.abstract | In metabolic engineering, enhanced production of value-added chemicals requires precise flux control between growth-essential competing and production pathways. Although advances in synthetic biology have facilitated the exploitation of a number of genetic elements for precise flux control, their use requires expensive inducers, or more importantly, needs complex and time-consuming processes to design and optimize appropriate regulator components, case-by-case. To overcome this issue, we devised the plug-in repressor libraries for target-specific flux control, in which expression levels of the repressors were diversified using degenerate 5' untranslated region (5' UTR) sequences employing the UTR Library Designer. After we validated a wide expression range of the repressor libraries, they were applied to improve the production of lycopene from glucose and 3-hydroxypropionic acid (3-HP) from acetate in Escherichia coli via precise flux re-balancing to enlarge precursor pools. Consequently, we successfully achieved optimal carbon fluxes around the precursor nodes for efficient production. The most optimized strains were observed to produce 2.59 g/L of 3-HP and 11.66 mg/L of lycopene, which were improved 16.5-fold and 2.82-fold, respectively, compared to those produced by the parental strains. These results indicate that carbon flux rebalancing using the plug-in library is a powerful strategy for efficient production of value-added chemicals in E. coli. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | METABOLIC ENGINEERING | - |
dc.title | Plug-in repressor library for precise regulation of metabolic flux in Escherichia coli | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.ymben.2021.07.013 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | METABOLIC ENGINEERING, v.67, pp.365 - 372 | - |
dc.identifier.wosid | 000694714100001 | - |
dc.citation.endPage | 372 | - |
dc.citation.startPage | 365 | - |
dc.citation.title | METABOLIC ENGINEERING | - |
dc.citation.volume | 67 | - |
dc.contributor.affiliatedAuthor | Lee, Ji Yeon | - |
dc.contributor.affiliatedAuthor | Cha, Sanghak | - |
dc.contributor.affiliatedAuthor | Lee, Ji Hoon | - |
dc.contributor.affiliatedAuthor | Lim, Hyun Gyu | - |
dc.contributor.affiliatedAuthor | Noh, Myung Hyun | - |
dc.contributor.affiliatedAuthor | Kang, Chae Won | - |
dc.contributor.affiliatedAuthor | Jung, Gyoo Yeol | - |
dc.identifier.scopusid | 2-s2.0-85111679885 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | MALONYL-COA PATHWAY | - |
dc.subject.keywordPlus | CONTROL GENE-EXPRESSION | - |
dc.subject.keywordPlus | 3-HYDROXYPROPIONIC ACID | - |
dc.subject.keywordPlus | FATTY-ACID | - |
dc.subject.keywordPlus | CARBON-FLUX | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | PROMOTERS | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Carbon flux rebalancing | - |
dc.subject.keywordAuthor | 3-Hydroxypropionic acid | - |
dc.subject.keywordAuthor | Lycopene | - |
dc.subject.keywordAuthor | UTR Library designer | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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