DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bai, Xilin | - |
dc.contributor.author | Liu, Yajie | - |
dc.contributor.author | Lee, Jiyeon | - |
dc.contributor.author | Fang, Jing | - |
dc.contributor.author | Wu, Wen-Hao | - |
dc.contributor.author | SEO, JONGCHEOL | - |
dc.contributor.author | Zhang, Wen-Bin | - |
dc.date.accessioned | 2022-02-28T06:40:07Z | - |
dc.date.available | 2022-02-28T06:40:07Z | - |
dc.date.created | 2022-02-24 | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 2666-5425 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/109580 | - |
dc.description.abstract | Topology has been recognized as a unique dimension in molecular engineering, yet the topological diversity remains largely untapped, especially in macromolecules. Herein, we report the molecular design, cellular synthesis, and detailed characterization of protein pretzelanes with a chemical topology of a bridged Hopf link. The synergy between the intramolecular chain entwining guided by the p53dim (X) domains and the genetically encoded side-chain coupling by SpyTag(A)-SpyCatcher(B) reaction facilitates the direct synthesis of the model protein pretzelane BXA-BXA in Escherichia coli. The approach tolerates the insertion of various proteins-of-interest, such as elastin-like protein (ELP), superfolder green fluorescent protein (GFP) and dihydrofolate reductase (DHFR), at the bridge region between two rings, giving rise to three protein pretzelanes BXA-ELP-BXA, BXA-GFP-BXA, and BXA-DHFR-BXA. Their topology has been verified by combined techniques of MALDI-TOF mass spectrometry, ion mobility-mass spectrometry, site-specific mutation, and orthogonal proteolytic digestion experiments. Not only are the fluorescent properties of GFP and the catalytic properties of DHFR fully retained, the pretzelane topology also renders BXA-DHFR-BXA more thermally resilient than the wild-type DHFR. These results expand the topological diversity of proteins and demonstrate protein stabilization as a potential functional benefit for the pretzelane topology. | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | Giant | - |
dc.title | Cellular Synthesis of Protein Pretzelanes | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.giant.2022.100092 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Giant, v.10 | - |
dc.citation.title | Giant | - |
dc.citation.volume | 10 | - |
dc.contributor.affiliatedAuthor | SEO, JONGCHEOL | - |
dc.identifier.scopusid | 2-s2.0-85124986983 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Catenane | - |
dc.subject.keywordAuthor | p53 | - |
dc.subject.keywordAuthor | P53 | - |
dc.subject.keywordAuthor | Pretzelane | - |
dc.subject.keywordAuthor | Protein | - |
dc.subject.keywordAuthor | SpyCatcher | - |
dc.subject.keywordAuthor | SpyTag | - |
dc.subject.keywordAuthor | Topology | - |
dc.description.journalRegisteredClass | scopus | - |
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