Establishment of in vitro multi-organ system using 3D bioprinting strategies for understanding molecular pathogenesis of enteric hyperoxaluria

Abstract

Enteric hyperoxaluria (a.k.a. secondary hyperoxaluria; SH) can occur as a complication of inflammatory bowel disease, causing oxalate malabsorption. The SH patients often have an increased risk of having recurrent kidney stones and loss of kidney function from oxalate nephropathy. Current therapeutic options are simply limited to correcting the underlying gastrointestinal disorders. Therefore developing SH model is needed to better define the precise factors that influence risk of having SH. In this study, in vitro SH model was successfully designed and constructed by utilizing 3D co-axial cell printing technique and transwell systems with integrated intestinal barrier and proximal tubule into a single platform. The hallmarks in SH pathogenesis have been successfully recapitulated on in vitro SH model, and the overall performance of this platform is being measured by multiple biochemical methods.