DC Field | Value | Language |
---|---|---|
dc.contributor.author | 안경익 | - |
dc.contributor.author | 차준영 | - |
dc.contributor.author | Lee Jeong Won | - |
dc.contributor.author | Park Gyeongran | - |
dc.contributor.author | 신경임 | - |
dc.contributor.author | Song Shi-Jian | - |
dc.contributor.author | Ryu Gyeongryul | - |
dc.contributor.author | 황인환 | - |
dc.contributor.author | 김민갑 | - |
dc.contributor.author | 김외연 | - |
dc.date.accessioned | 2022-03-03T08:40:19Z | - |
dc.date.available | 2022-03-03T08:40:19Z | - |
dc.date.created | 2022-03-01 | - |
dc.date.issued | 2022-02 | - |
dc.identifier.issn | 1226-9239 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/110306 | - |
dc.description.abstract | Viral diseases are extremely widespread infections that change constantly through mutations. To produce vaccines against viral diseases, transient expression systems are employed, and Nicotiana benthamiana (tobacco) plants are a rapidly expanding platform. In this study, we developed a recombinant protein vaccine targeting the major capsid protein (MCP) of iridovirus fused with the lysine motif (LysM) and coiled-coil domain of coronin 1 (ccCor1) for surface display using Lactococcus lactis. The protein was abundantly produced in N. benthamiana in its N-glycosylated form. Total soluble proteins isolated from infiltrated N. benthamiana leaves were treated sequentially with increasing ammonium sulfate solution, and recombinant MCP mainly precipitated at 40–60%. Additionally, affinity chromatography using Ni–NTA resin was applied for further purification. Native structure analysis using size exclusion chromatography showed that recombinant MCP existed in a large oligomeric form. A minimum OD600 value of 0.4 trichloroacetic acid (TCA)-treated L. lactis was required for efficient recombinant MCP display. Immunogenicity of recombinant MCP was assessed in a mouse model through enzyme-linked immunosorbent assay (ELISA) with serum-injected recombinant MCP-displaying L. lactis. In summary, we developed a plant-based recombinant vaccine production system combined with surface display on L. lactis. | - |
dc.language | English | - |
dc.publisher | 한국식물학회 | - |
dc.relation.isPartOf | Journal of Plant Biology | - |
dc.title | Production of a Bacteria-like Particle Vaccine Targeting Rock Bream (Oplegnathus fasciatus) Iridovirus Using Nicotiana benthamiana | - |
dc.title.alternative | Production of a Bacteria-like Particle Vaccine Targeting Rock Bream (Oplegnathus fasciatus) Iridovirus Using Nicotiana benthamiana | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12374-021-09328-z | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Journal of Plant Biology, v.65, no.1, pp.21 - 28 | - |
dc.identifier.kciid | ART002809060 | - |
dc.identifier.wosid | 000701797400001 | - |
dc.citation.endPage | 28 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 21 | - |
dc.citation.title | Journal of Plant Biology | - |
dc.citation.volume | 65 | - |
dc.contributor.affiliatedAuthor | Song Shi-Jian | - |
dc.contributor.affiliatedAuthor | 황인환 | - |
dc.identifier.scopusid | 2-s2.0-85115849034 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RED-SEA BREAM | - |
dc.subject.keywordPlus | MAJOR CAPSID PROTEIN | - |
dc.subject.keywordPlus | GROUPER IRIDOVIRUS | - |
dc.subject.keywordPlus | INFECTIOUS SPLEEN | - |
dc.subject.keywordPlus | CULTURED FISH | - |
dc.subject.keywordPlus | PAGRUS-MAJOR | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | TEMMINCK | - |
dc.subject.keywordPlus | MOTIF | - |
dc.subject.keywordAuthor | Bacteria-like particle Iridovirus Major capsid protein Nicotiana benthamiana Plant-based vaccine | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Plant Sciences | - |
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