노화를 조절하는 long non-coding RNA 규명

Abstract

Recent studies indicate that long non-coding RNA (lncRNA) play modulatory roles in various physiological processes. However, the functional significance of lncRNAs in lifespan regulation remained largely unknown. In this study, we aimed at identification of C. elegans lncRNAs that modulate lifespan. We found that 4 lncRNAs out of tested 81 lncRNAs, linc-98, linc-100, linc-101, and linc-107, were required for the longevity of animals with reduced insulin/IGF-1 signaling (IIS), a conserved aging-regulating pathway. We also showed that the genetic inhibition of each of 4 lncRNAs, linc-8, linc-115, linc-120, and linc-123, extended lifespan in C. elegans. We will continue the lifespan screen and will generate lincRNA gene knockout animals using CRISPR/Cas9 system. Overall, this study will provide insights into how lncRNAs contribute to organismal aging and longevity. Furthermore, this research will help understanding the biology of noncoding RNAs as novel biomarkers or drug targets for aging research.