Open Access System for Information Sharing

Login Library

 

Article
Cited 118 time in webofscience Cited 136 time in scopus
Metadata Downloads

Microfabrication-based modulation of embryonic stem cell differentiation SCIE SCOPUS

Title
Microfabrication-based modulation of embryonic stem cell differentiation
Authors
Park, JCho, CHParashurama, NLi, YWBerthiaume, FToner, MTilles, AWYarmush, ML
Date Issued
2007-01
Publisher
ROYAL SOC CHEMISTRY
Abstract
Embryonic stem (ES) cells form spontaneous aggregates during differentiation, and cell-cell communication in the aggregates plays an important role in differentiation. The development of a controlled differentiation scheme for ES cells has been hindered by the lack of a reliable method to produce uniform aggregate sizes. Conventional techniques, such as hanging drop and suspension cultures, do not allow precise control over size of ES cell aggregates. To surmount this problem, we microfabricated adhesive stencils to make mouse ES (mES) cell aggregates of specific sizes ranging from 100 mu m to 500 mu m in diameter. With this technique, we studied the effect of the initial aggregate size on ES cell differentiation. After 20 days of induction of differentiation, we analyzed the stem cell populations using gene and protein expression assays as well as biochemical functions. Notably, we found that germ layer differentiation depends on the initial size of the ES cell aggregate. Among the ES cell aggregate sizes tested, the aggregates with 300 mu m diameter showed similar differentiation profiles of three germ layers as embryoid bodies made using the "hanging drop'' technique. The smaller (100 mu m) aggregates showed the increased expression of ectodermal markers compared to the larger (500 mu m) aggregates, while the 500 mu m aggregates showed the increased expression of mesodermal and endodermal markers compared to the 100 mu m aggregates. These results indicate that the initial size of the aggregate is an important factor for ES cell differentiation, and can affect germ layer selection as well as the extent of differentiation.
URI
https://oasis.postech.ac.kr/handle/2014.oak/11320
DOI
10.1039/B704739H
ISSN
1473-0197
Article Type
Article
Citation
LAB ON A CHIP, vol. 7, no. 8, page. 1018 - 1028, 2007-01
Files in This Item:

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

박재성PARK, JAE SUNG
Dept of Mechanical Enginrg
Read more

Views & Downloads

Browse