DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, H.J. | - |
dc.contributor.author | Kim, B.H. | - |
dc.date.accessioned | 2022-06-29T10:20:59Z | - |
dc.date.available | 2022-06-29T10:20:59Z | - |
dc.date.created | 2021-12-19 | - |
dc.date.issued | 2021-02 | - |
dc.identifier.issn | 2576-6422 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/113264 | - |
dc.description.abstract | MicroRNAs (miRNAs) regulate gene expression in cells; high levels of expression are associated with various cancers. In this paper, we describe PyA-modified nucleic acid probes that can detect intracellular miRNAs by forming DNA/RNA hybrid three-way junction structures containing a fluorescent scaffold - a so-called G-cluster. This G-cluster featured two mismatched strands, four guanine residues, and one fluorescent adenine residue having a pyrene moiety covalently connected at the 8-position through an acetylene linker. The scaffold underwent a dramatic shift in its emission wavelength when two mismatched strands formed a duplex, similar to the behavior of an adenine pentad system (A-cluster). We applied the G-cluster scaffold in a three-way junction system to probe for miRNAs; its red-shifted fluorescence intensity and stability were greater than those reported previously for A-cluster three-way junction probes. Furthermore, confocal microscopy of cancer cell lines revealed bright fluorescence emissions in response to the miRNAs in the cells. Thus, this system can be applied intracellularly as a potential fluorescent probe for the detection of various biologically important nucleic acids. ? | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.relation.isPartOf | ACS Applied Bio Materials | - |
dc.title | Pyrene-Modified Guanine Cluster Probes Forming DNA/RNA Hybrid Three-Way Junctions for Imaging of Intracellular MicroRNAs | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acsabm.0c01476 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ACS Applied Bio Materials, v.4, no.2, pp.1668 - 1676 | - |
dc.citation.endPage | 1676 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 1668 | - |
dc.citation.title | ACS Applied Bio Materials | - |
dc.citation.volume | 4 | - |
dc.contributor.affiliatedAuthor | Lee, H.J. | - |
dc.contributor.affiliatedAuthor | Kim, B.H. | - |
dc.identifier.scopusid | 2-s2.0-85099938994 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | Cell culture | - |
dc.subject.keywordPlus | Diseases | - |
dc.subject.keywordPlus | Fluorescence | - |
dc.subject.keywordPlus | Gene expression | - |
dc.subject.keywordPlus | Positive ions | - |
dc.subject.keywordPlus | Pyrene | - |
dc.subject.keywordPlus | Red Shift | - |
dc.subject.keywordPlus | Cancer cell lines | - |
dc.subject.keywordPlus | Emission wavelength | - |
dc.subject.keywordPlus | Fluorescence emission | - |
dc.subject.keywordPlus | Fluorescent probes | - |
dc.subject.keywordPlus | Junction probes | - |
dc.subject.keywordPlus | Junction structure | - |
dc.subject.keywordPlus | Modified guanine | - |
dc.subject.keywordPlus | Red-shifted fluorescence | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordAuthor | confocal microscopy | - |
dc.subject.keywordAuthor | fluorescent probe | - |
dc.subject.keywordAuthor | guanine | - |
dc.subject.keywordAuthor | miRNA detection | - |
dc.subject.keywordAuthor | three-way junction | - |
dc.description.journalRegisteredClass | scopus | - |
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