Extended depth-of-field microscopy with a micro-mirror array lens system for versatile cellular examination

Abstract

Extended depth of field microscopy is a method to acquire complete object image in a single view, but they have their respective limitations. We present a versatile EDOF wide-field microscopy that insensitive to calibration and orientation by using a micro-mirror array lens system (MALS), a MEMS-based fast dynamic varifocal lens. The system performance was demonstrated in two imaging cases: imaging of conjunctival surface of live mouse eye and surface cell examination of human tissues.