DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Keondo | - |
dc.contributor.author | Lee, Donghyeon | - |
dc.contributor.author | Park, Jaehyun | - |
dc.contributor.author | Lee, Jong-Lam | - |
dc.contributor.author | Chung, Wan Kyun | - |
dc.contributor.author | Cho, Yunje | - |
dc.contributor.author | Nam, Ki Hyun | - |
dc.date.accessioned | 2023-01-06T02:20:13Z | - |
dc.date.available | 2023-01-06T02:20:13Z | - |
dc.date.created | 2022-11-04 | - |
dc.date.issued | 2022-09 | - |
dc.identifier.issn | 2076-3417 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/114872 | - |
dc.description.abstract | © 2022 by the authors.Featured Application: Recently upgraded inject-and-transfer system will improve beamtime efficiency for serial femtosecond crystallography. Serial femtosecond crystallography (SFX) using an X-ray free-electron laser (XFEL) can be applied to determine the room-temperature structure of target molecules while minimizing radiation damage and visualizing molecular dynamics. In SFX, a sample delivery system is required to deliver microcrystals to the XFEL beam path in a serial manner. We recently developed a sample delivery method, the combined inject-and-transfer system (BITS), which is a hybrid method based on the injector and fixed-target scanning approach. In this study, we introduced recently upgraded hardware to move the injection needle in the direction of the XYZ-axis and a graphic user interface for user motion control. Furthermore, we report that the viscous solution containing 10% (w/v) PEG 3350 or PEG 6000 that is widely used for protein crystallization can be stably deposited on polyimide film with a hydrophobic surface without any special treatment. Moreover, the development of an inject-and-diffuse method for time-resolved studies with liquid applications in the BITS and its preliminary results are reported. This study provides up-to-date instrument information to SFX users using BITS and provides insights to instrument developers for SFX. | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | Applied Sciences-basel | - |
dc.title | Upgraded Combined Inject-and-Transfer System for Serial Femtosecond Crystallography | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/app12189125 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Applied Sciences-basel, v.12, no.18 | - |
dc.identifier.wosid | 000857610800001 | - |
dc.citation.number | 18 | - |
dc.citation.title | Applied Sciences-basel | - |
dc.citation.volume | 12 | - |
dc.contributor.affiliatedAuthor | Lee, Keondo | - |
dc.contributor.affiliatedAuthor | Lee, Donghyeon | - |
dc.contributor.affiliatedAuthor | Park, Jaehyun | - |
dc.contributor.affiliatedAuthor | Lee, Jong-Lam | - |
dc.contributor.affiliatedAuthor | Chung, Wan Kyun | - |
dc.contributor.affiliatedAuthor | Cho, Yunje | - |
dc.contributor.affiliatedAuthor | Nam, Ki Hyun | - |
dc.identifier.scopusid | 2-s2.0-85138662705 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | BITS | - |
dc.subject.keywordAuthor | fixed-target | - |
dc.subject.keywordAuthor | injection | - |
dc.subject.keywordAuthor | mix-and-inject | - |
dc.subject.keywordAuthor | sample chamber | - |
dc.subject.keywordAuthor | serial femtosecond crystallography | - |
dc.subject.keywordAuthor | X-ray free-electron laser | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Engineering, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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