DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Do Soon | - |
dc.contributor.author | Watkins, Andrew | - |
dc.contributor.author | Bidstrup, Erik | - |
dc.contributor.author | Lee, Joongoo | - |
dc.contributor.author | Topkar, Ved | - |
dc.contributor.author | Kofman, Camila | - |
dc.contributor.author | Schwarz, Kevin J. | - |
dc.contributor.author | Liu, Yan | - |
dc.contributor.author | Pintilie, Grigore | - |
dc.contributor.author | Roney, Emily | - |
dc.contributor.author | Das, Rhiju | - |
dc.contributor.author | Jewett, Michael C. | - |
dc.date.accessioned | 2023-03-02T06:41:06Z | - |
dc.date.available | 2023-03-02T06:41:06Z | - |
dc.date.created | 2023-03-01 | - |
dc.date.issued | 2022-09 | - |
dc.identifier.issn | 1552-4450 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/116188 | - |
dc.description.abstract | RNA-based macromolecular machines, such as the ribosome, have functional parts reliant on structural interactions spanning sequence-distant regions. These features limit evolutionary exploration of mutant libraries and confound three-dimensional structure-guided design. To address these challenges, we describe Evolink (evolution and linkage), a method that enables high-throughput evolution of sequence-distant regions in large macromolecular machines, and library design guided by computational RNA modeling to enable exploration of structurally stable designs. Using Evolink, we evolved a tethered ribosome with a 58% increased activity in orthogonal protein translation and a 97% improvement in doubling times in SQ171 cells compared to a previously developed tethered ribosome, and reveal new permissible sequences in a pair of ribosomal helices with previously explored biological function. The Evolink approach may enable enhanced engineering of macromolecular machines for new and improved functions for synthetic biology. [Figure not available: see fulltext.] © 2022, The Author(s), under exclusive licence to Springer Nature America, Inc. | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | Nature Chemical Biology | - |
dc.title | Three-dimensional structure-guided evolution of a ribosome with tethered subunits | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41589-022-01064-w | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Nature Chemical Biology, v.18, no.9, pp.990 - 998 | - |
dc.identifier.wosid | 000825247900002 | - |
dc.citation.endPage | 998 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 990 | - |
dc.citation.title | Nature Chemical Biology | - |
dc.citation.volume | 18 | - |
dc.contributor.affiliatedAuthor | Lee, Joongoo | - |
dc.identifier.scopusid | 2-s2.0-85134316976 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | AMINO-ACIDS | - |
dc.subject.keywordPlus | PROTEIN-SYNTHESIS | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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