DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Shi‐Jian | - |
dc.contributor.author | Kim, Heeyeon | - |
dc.contributor.author | Jang, Eun Young | - |
dc.contributor.author | Jeon, Hyungmin | - |
dc.contributor.author | Diao, Hai‐Ping | - |
dc.contributor.author | Khan, Md Rezaul Islam | - |
dc.contributor.author | Lee, Mi‐Seon | - |
dc.contributor.author | Lee, Young Jae | - |
dc.contributor.author | Nam, Jeong‐hyun | - |
dc.contributor.author | Kim, Seong‐Ryeol | - |
dc.contributor.author | Kim, Young‐Jin | - |
dc.contributor.author | Sohn, Eun‐Ju | - |
dc.contributor.author | Hwang, Inhwan | - |
dc.contributor.author | Choi, Jang‐Hoon | - |
dc.date.accessioned | 2023-03-02T09:00:25Z | - |
dc.date.available | 2023-03-02T09:00:25Z | - |
dc.date.created | 2023-03-02 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 1467-7644 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/116301 | - |
dc.description.abstract | The ongoing coronavirus disease 2019 (COVID-19) pandemic has spurred rapid development of vaccines as part of the public health response. However, the general strategy used to construct recombinant trimeric severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) proteins in mammalian cells is not completely adaptive to molecular farming. Therefore, we generated several constructs of recombinant S proteins for high expression in Nicotiana benthamiana. Intramuscular injection of N. benthamiana-expressed S-ct vaccine (NS(ct)Vac) into Balb/c mice elicited both humoral and cellular immune responses, and booster doses increased neutralizing antibody titres. In human angiotensin-converting enzyme knock-in mice, two doses of NS(ct)Vac induced anti-S and neutralizing antibodies, which cross-neutralized Alpha, Beta, Delta and Omicron variants. Survival rates after lethal challenge with SARS-CoV-2 were up to 80%, without significant body weight loss, and viral titres in lung tissue fell rapidly, with no infectious virus detectable at 7-day post-infection. Thus, plant-derived NS(ct)Vac could be a candidate COVID-19 vaccine. | - |
dc.language | English | - |
dc.publisher | Blackwell Publishing Inc. | - |
dc.relation.isPartOf | Plant Biotechnology Journal | - |
dc.title | SARS-CoV-2 spike trimer vaccine expressed in Nicotiana benthamiana adjuvanted with Alum elicits protective immune responses in mice | - |
dc.type | Article | - |
dc.identifier.doi | 10.1111/pbi.13908 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Plant Biotechnology Journal, v.20, no.12, pp.2298 - 2312 | - |
dc.identifier.wosid | 000849822600001 | - |
dc.citation.endPage | 2312 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2298 | - |
dc.citation.title | Plant Biotechnology Journal | - |
dc.citation.volume | 20 | - |
dc.contributor.affiliatedAuthor | Hwang, Inhwan | - |
dc.identifier.scopusid | 2-s2.0-85137325631 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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