DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mok, Jihyun | - |
dc.contributor.author | Kim, Eunseon | - |
dc.contributor.author | Kang, Minji | - |
dc.contributor.author | Jeon, Jinseong | - |
dc.contributor.author | Ban, Changill | - |
dc.date.accessioned | 2023-03-06T00:00:28Z | - |
dc.date.available | 2023-03-06T00:00:28Z | - |
dc.date.created | 2023-02-02 | - |
dc.date.issued | 2023-02 | - |
dc.identifier.issn | 0039-9140 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/116809 | - |
dc.description.abstract | © 2022Here, we proposed an enzyme-linked oligonucleotide assay (ELONA) for yellow fever (YF) diagnosis that uses a pair of aptamers, YFns1-4 and YFns1-31. The aptamers were selected to specifically bind to nonstructural protein 1 (NS1), which is secreted at a high concentration after YF infection. We applied the aptamers which did not interfere with each other on binding to the NS1 in a sandwich ELONA. In the assay, the best detection sensitivity was obtained when the combination of YFns1-31 as a capture aptamer and YFns1-4 as a detect aptamer was used. The sensitivity could be attributed to the results of the direct ELONA with each YFns1-4 and YFns1-31; a great absorbance intensity and a broad detectable range of NS1, respectively. The sandwich ELONA achieved a low detection limit of 0.85 nM in buffer and was highly specific to the YFV-NS1 as its detection signals were significantly distinct from those of other flavivirus-derived NS1. In addition, the assay showed a desirable sensitivity in serum-spiked condition. Our developed sandwich ELONA can be a new practical and applicable serological diagnostics in YF endemic regions where other flaviviruses coexist and facilities for complex diagnostic tests are lacking. | - |
dc.language | English | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isPartOf | Talanta | - |
dc.title | Development of an optical sandwich ELONA using a pair of DNA aptamers for yellow fever virus NS1 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.talanta.2022.123979 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Talanta, v.253 | - |
dc.citation.title | Talanta | - |
dc.citation.volume | 253 | - |
dc.contributor.affiliatedAuthor | Mok, Jihyun | - |
dc.contributor.affiliatedAuthor | Kim, Eunseon | - |
dc.contributor.affiliatedAuthor | Kang, Minji | - |
dc.contributor.affiliatedAuthor | Jeon, Jinseong | - |
dc.contributor.affiliatedAuthor | Ban, Changill | - |
dc.identifier.scopusid | 2-s2.0-85139329135 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Aptamer | - |
dc.subject.keywordAuthor | Flavivirus | - |
dc.subject.keywordAuthor | Non-structural 1 protein | - |
dc.subject.keywordAuthor | Sandwich ELONA | - |
dc.subject.keywordAuthor | Serological diagnostics | - |
dc.subject.keywordAuthor | Yellow fever virus | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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