Flux optimization for improved caproic acid production in Escherichia coli
- Title
- Flux optimization for improved caproic acid production in Escherichia coli
- Authors
- NAM, SEONG HYEON; KIM, SEONG GYEONG; JANG, SUNGHO; JAE, HYUNG LIM; JUNG, GYOO YEOL
- Date Issued
- 2023-05-11
- Publisher
- 한국공업화학회
- Abstract
- The purpose of this study is to demonstrate that rebalancing of metabolic fluxes at acetyl-CoA branch node can substantially improve the titer and productivity of hexanoic acid in recombinant Escherichia coli strains. First, a hexanoic acid-producing E. coli strain was constructed by expressing genes encoding β-ketothiolase from Cupriavidus necator and acetyl-CoA transferase from Megasphaera sp. MH in a butyric acid producer strain. Next, metabolic flux was optimized at the acetyl-CoA branch node by fine-tuning the expression level of the gene for acetyl-CoA acetyltransferase. Four synthetic 5′-UTR regions were designed for atoB using UTR Designer to modulate the expression level of the gene. Notably, the productivity of the optimized strain (14.7 mg/L/h) was the highest among recombinant E. coli strains in literature when using a similar inoculum size for fermentation. These results show that fine-tuning the expression level of atoB is critical for production of hexanoic acid.
Keywords: Flux optimization, hexanoic acid, metabolic engineering
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/117671
- Article Type
- Conference
- Citation
- 2023 한국공업화학회 춘계 총회 및 학술대회, 2023-05-11
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