Regulation of Capicua function by Extracellular signal-regulated kinase
- Title
- Regulation of Capicua function by Extracellular signal-regulated kinase
- Authors
- 박종민
- Date Issued
- 2023
- Publisher
- 포항공과대학교
- Abstract
- Activation of receptor tyrosine kinase (RTK) signaling inactivates capicua (CIC), a transcriptional repressor that functions as a tumor suppressor, via degradation and/or cytoplasmic translocation. Although CIC is known to be inactivated by phosphorylation, the mechanisms underlying the cytoplasmic translocation of CIC remain poorly understood. Therefore, I aimed to evaluate the roles of extracellular signal-regulated kinase (ERK), p90RSK, and c-SRC in the epidermal growth factor receptor (EGFR) activation-induced cytoplasmic translocation of CIC and further investigated the molecular mechanisms for this regulation. I discovered that nuclear ERK most effectively induced nuclear export of CIC-S. I identified 12 serine and threonine (S/T) residues within CIC, including S173 and S301 residues that are phosphorylated by p90RSK, which contribute to the cytoplasmic translocation of CIC-S when phosphorylated. The amino-terminal (CIC-S-N) and carboxyl-terminal (CIC-S-C) regions of CIC-S were found to interact with each other to promote their nuclear localization. EGF treatment disrupted the interaction between CIC-S-N and CIC-S-C and induced their cytoplasmic translocation. Furthermore, phosphorylation of CIC-S by activated ERK significantly reduced the DNA binding affinity of CIC-S in vitro. Alanine substitution for the 12 S/T residues blocked the cytoplasmic translocation of CIC-S and consequently enhanced the tumor suppressor activity of CIC-S.
My findings demonstrate that ERK-mediated disruption of intramolecular interaction of CIC is critical for the cytoplasmic translocation of CIC and dissociation CIC from DNA. Notably, my findings suggest that the nuclear retention of CIC may represent a strategy for cancer therapy.
- URI
- http://postech.dcollection.net/common/orgView/200000660899
https://oasis.postech.ac.kr/handle/2014.oak/118285
- Article Type
- Thesis
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.