Cationic nucleolipids as efficient siRNA carriers

Abstract

We synthesized five novel uridine-based cationic nucleolipids, introducing basic amino acid residues at the 5 ' position of uridine, through 1,3-dipolar cycloaddition, and hydrophobic alkyl moieties at the 2 ' and 3 ' positions, through carbamate linkages. Their lipoplexes delivered siRNAs efficiently to cells, in vitro, without any severe toxicity.