DC Field | Value | Language |
---|---|---|
dc.contributor.author | HYERI, YUN | - |
dc.contributor.author | Minkyo Jung | - |
dc.contributor.author | HOJIN, LEE | - |
dc.contributor.author | Sungjin Jung | - |
dc.contributor.author | KIM, TAEHYEON | - |
dc.contributor.author | KIM, NARI | - |
dc.contributor.author | PARK, SEUNG YEOL | - |
dc.contributor.author | KIM, WON JONG | - |
dc.contributor.author | Ji Young Mun | - |
dc.contributor.author | YOO, JOO YEON | - |
dc.date.accessioned | 2024-02-29T05:00:24Z | - |
dc.date.available | 2024-02-29T05:00:24Z | - |
dc.date.created | 2024-02-28 | - |
dc.date.issued | 2023-08 | - |
dc.identifier.issn | 1469-221X | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/120541 | - |
dc.description.abstract | The ER regulates the spatiotemporal organization of endolysosomal systems by membrane contact. In addition to tethering via heterotypic interactions on both organelles, we present a novel ER-endosome tethering mechanism mediated by homotypic interactions. The single-pass transmembrane protein SCOTIN is detected in the membrane of the ER and endosomes. In SCOTIN-knockout (KO) cells, the ER-late endosome contacts are reduced, and the perinuclear positioning of endosomes is disturbed. The cytosolic proline-rich domain (PRD) of SCOTIN forms homotypic assemblies in vitro and is necessary for ER-endosome membrane tethering in cells. A region of 28 amino acids spanning 150–177 within the SCOTIN PRD is essential to elicit membrane tethering and endosomal dynamics, as verified by reconstitution in SCOTIN-KO cells. The assembly of SCOTIN (PRD) is sufficient to mediate membrane tethering, as purified SCOTIN (PRD), but not SCOTIN (PRDΔ150-177), brings two different liposomes closer in vitro. Using organelle-specific targeting of a chimeric PRD domain shows that only the presence on both organellar membranes enables the ER-endosome membrane contact, indicating that the assembly of SCOTIN on heterologous membranes mediates organelle tethering. © 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license. | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | EMBO Reports | - |
dc.title | Homotypic SCOTIN assemblies form ER-endosome membrane contacts and regulate endosome dynamics | - |
dc.type | Article | - |
dc.identifier.doi | 10.15252/embr.202256538 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | EMBO Reports, v.24, no.8 | - |
dc.identifier.wosid | 001017716400001 | - |
dc.citation.number | 8 | - |
dc.citation.title | EMBO Reports | - |
dc.citation.volume | 24 | - |
dc.contributor.affiliatedAuthor | HYERI, YUN | - |
dc.contributor.affiliatedAuthor | HOJIN, LEE | - |
dc.contributor.affiliatedAuthor | KIM, TAEHYEON | - |
dc.contributor.affiliatedAuthor | KIM, NARI | - |
dc.contributor.affiliatedAuthor | PARK, SEUNG YEOL | - |
dc.contributor.affiliatedAuthor | KIM, WON JONG | - |
dc.contributor.affiliatedAuthor | YOO, JOO YEON | - |
dc.identifier.scopusid | 2-s2.0-85163303507 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | SITES | - |
dc.subject.keywordPlus | TETHER | - |
dc.subject.keywordPlus | MATURATION | - |
dc.subject.keywordAuthor | endoplasmic reticulum | - |
dc.subject.keywordAuthor | endosome | - |
dc.subject.keywordAuthor | membrane contact | - |
dc.subject.keywordAuthor | SCOTIN | - |
dc.subject.keywordAuthor | self-assembly | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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