DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Ae Sol | - |
dc.contributor.author | KIm, Su Min | - |
dc.contributor.author | KIm, Kyeong Rok | - |
dc.contributor.author | Park, Chulmin | - |
dc.contributor.author | Lee, Dong-Gun | - |
dc.contributor.author | Heo, Hye Ryoung | - |
dc.contributor.author | Cha, Hyung Joon | - |
dc.contributor.author | Kim, Chang Sup | - |
dc.date.accessioned | 2024-03-04T07:43:35Z | - |
dc.date.available | 2024-03-04T07:43:35Z | - |
dc.date.created | 2024-03-01 | - |
dc.date.issued | 2023-03 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/120748 | - |
dc.description.abstract | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19). The high human-to-human transmission and rapid evolution of SARS-CoV-2 have resulted in a worldwide pandemic. To contain SARS-CoV-2, it is essential to efficiently control the transmission of the virus through the early diagnosis of infected individuals, including asymptomatic people. Therefore, a rapid and accurate assay is vital for the early diagnosis of SARS-CoV-2 in suspected individuals. In this study, we developed a colorimetric lateral flow immunoassay (LFIA) in which a CBP31-BC linker was used to immobilize antibodies on a cellulose membrane in an oriented manner. The developed LFIA enabled sensitive detection of cultured SARS-CoV-2 in 15 min with a detection limit of 5 x 104 copies/mL. The clinical performance of the LFIA for detecting SARS-CoV-2 was evaluated using 19 clinical samples validated by reverse transcription-polymerase chain reaction (RT-PCR). The LFIA detected all the positive and negative samples accurately, corresponding to 100% accuracy. Impor-tantly, patient samples with low viral loads were accurately identified. Thus, the proposed method can provide a useful platform for rapid and accurate point-of-care testing of SARS-CoV-2 in infected individuals to efficiently control the COVID-19 pandemic. | - |
dc.language | English | - |
dc.publisher | Elsevier BV | - |
dc.relation.isPartOf | Sensors and Actuators, B: Chemical | - |
dc.title | A colorimetric lateral flow immunoassay based on oriented antibody immobilization for sensitive detection of SARS-CoV-2 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.snb.2022.133245 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Sensors and Actuators, B: Chemical, v.379 | - |
dc.identifier.wosid | 000972029100001 | - |
dc.citation.title | Sensors and Actuators, B: Chemical | - |
dc.citation.volume | 379 | - |
dc.contributor.affiliatedAuthor | Cha, Hyung Joon | - |
dc.identifier.scopusid | 2-s2.0-85145003761 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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