DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, YW | - |
dc.contributor.author | Chung, YJ | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Lee, HJ | - |
dc.contributor.author | Park, J | - |
dc.contributor.author | Roh, TY | - |
dc.contributor.author | Cho, HJ | - |
dc.contributor.author | Yoon, CH | - |
dc.contributor.author | Koo, BK | - |
dc.contributor.author | Kim, HS | - |
dc.date.accessioned | 2015-06-25T03:25:55Z | - |
dc.date.available | 2015-06-25T03:25:55Z | - |
dc.date.created | 2014-02-14 | - |
dc.date.issued | 2014-01-22 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.other | 2015-OAK-0000028833 | en_US |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/12707 | - |
dc.description.abstract | In patients with Parkinson's disease (PD), stem cells can serve as therapeutic agents to restore or regenerate injured nervous system. Here, we differentiated two types of stem cells; mouse embryonic stem cells (mESCs) and protein-based iPS cells (P-iPSCs) generated by non-viral methods, into midbrain dopaminergic (mDA) neurons, and then compared the efficiency of DA neuron differentiation from these two cell types. In the undifferentiated stage, P-iPSCs expressed pluripotency markers as ES cells did, indicating that protein-based reprogramming was stable and authentic. While both stem cell types were differentiated to the terminally-matured mDA neurons, P-iPSCs showed higher DA neuron-specific markers' expression than ES cells. To investigate the mechanism of the superior induction capacity of DA neurons observed in P-iPSCs compared to ES cells, we analyzed histone modifications by genome-wide ChIP sequencing analysis and their corresponding microarray results between two cell types. We found that Wnt signaling was up-regulated, while SFRP1, a counter-acting molecule of Wnt, was more suppressed in P-iPSCs than in mESCs. In PD rat model, transplantation of neural precursor cells derived from both cell types showed improved function. The present study demonstrates that P-iPSCs could be a suitable cell source to provide patient-specific therapy for PD without ethical problems or rejection issues. | - |
dc.description.statementofresponsibility | open | en_US |
dc.language | English | - |
dc.publisher | PLOS | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | BY_NC_ND | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.0/kr | en_US |
dc.title | Comparative Study of Efficacy of Dopaminergic Neuron Differentiation between Embryonic Stem Cell and Protein-Based Induced Pluripotent Stem Cell | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | en_US |
dc.identifier.doi | 10.1371/JOURNAL.PONE.0085736 | - |
dc.author.google | Kwon, YW | en_US |
dc.author.google | Chung, YJ | en_US |
dc.author.google | Kim, HS | en_US |
dc.author.google | Koo, BK | en_US |
dc.author.google | Yoon, CH | en_US |
dc.author.google | Cho, HJ | en_US |
dc.author.google | Roh, TY | en_US |
dc.author.google | Park, J | en_US |
dc.author.google | Lee, HJ | en_US |
dc.author.google | Kim, J | en_US |
dc.relation.volume | 9 | en_US |
dc.relation.issue | 1 | en_US |
dc.relation.startpage | E85736 | en_US |
dc.contributor.id | 10138348 | en_US |
dc.relation.journal | PLOS ONE | en_US |
dc.relation.index | SCI급, SCOPUS 등재논문 | en_US |
dc.relation.sci | SCI | en_US |
dc.collections.name | Journal Papers | en_US |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.9, no.1, pp.E85736 | - |
dc.identifier.wosid | 000330283100066 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | E85736 | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 9 | - |
dc.contributor.affiliatedAuthor | Roh, TY | - |
dc.identifier.scopusid | 2-s2.0-84899831985 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 9 | - |
dc.description.scptc | 11 | * |
dc.date.scptcdate | 2018-10-274 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PARKINSONS-DISEASE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ANIMAL-MODEL | - |
dc.subject.keywordPlus | RAT MODEL | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | MIDBRAIN | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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