DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gwon, GH | - |
dc.contributor.author | Jo, A | - |
dc.contributor.author | Baek, K | - |
dc.contributor.author | Jin, KS | - |
dc.contributor.author | Fu, Y | - |
dc.contributor.author | Lee, JB | - |
dc.contributor.author | Kim, Y | - |
dc.contributor.author | Cho, Y | - |
dc.date.accessioned | 2016-03-31T07:30:04Z | - |
dc.date.available | 2016-03-31T07:30:04Z | - |
dc.date.created | 2015-02-25 | - |
dc.date.issued | 2014-05-02 | - |
dc.identifier.issn | 0261-4189 | - |
dc.identifier.other | 2014-OAK-0000032045 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/13700 | - |
dc.description.abstract | The Mus81-Eme1 complex is a structure-selective endonuclease with a critical role in the resolution of recombination intermediates during DNA repair after interstrand cross-links, replication fork collapse, or double-strand breaks. To explain the molecular basis of 3 ' flap substrate recognition and cleavage mechanism by Mus81-Eme1, we determined crystal structures of human Mus81-Eme1 bound to various flap DNA substrates. Mus81-Eme1 undergoes gross substrate-induced conformational changes that reveal two key features: (i) a hydrophobic wedge of Mus81 that separates pre- and post-nick duplex DNA and (ii) a 5 ' end binding pocket that hosts the 5 ' nicked end of post-nick DNA. These features are crucial for comprehensive protein-DNA interaction, sharp bending of the 3 ' flap DNA substrate, and incision strand placement at the active site. While Mus81-Eme1 unexpectedly shares several common features with members of the 5 ' flap nuclease family, the combined structural, biochemical, and biophysical analyses explain why Mus81-Eme1 preferentially cleaves 3 ' flap DNA substrates with 5 ' nicked ends. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | EMBO Journal | - |
dc.title | Crystal Structures of the structure-selective nuclease Mus81-Eme1 bound to flap DNA substrates | - |
dc.type | Article | - |
dc.contributor.college | 물리학과 | - |
dc.identifier.doi | 10.1002/EMBJ.201487820 | - |
dc.author.google | Gwon, GH | - |
dc.author.google | Jo, A | - |
dc.author.google | Baek, K | - |
dc.author.google | Jin, KS | - |
dc.author.google | Fu, Y | - |
dc.author.google | Lee, JB | - |
dc.author.google | Kim, Y | - |
dc.author.google | Cho, Y | - |
dc.relation.volume | 33 | - |
dc.relation.issue | 9 | - |
dc.relation.startpage | 1061 | - |
dc.relation.lastpage | 1072 | - |
dc.contributor.id | 10171158 | - |
dc.relation.journal | EMBO JOURNAL | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | EMBO Journal, v.33, no.9, pp.1061 - 1072 | - |
dc.identifier.wosid | 000336495500011 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 1072 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1061 | - |
dc.citation.title | EMBO Journal | - |
dc.citation.volume | 33 | - |
dc.contributor.affiliatedAuthor | Lee, JB | - |
dc.contributor.affiliatedAuthor | Cho, Y | - |
dc.identifier.scopusid | 2-s2.0-84899814719 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 11 | - |
dc.description.scptc | 9 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | HOLLIDAY JUNCTION RESOLUTION | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE MUS81-MMS4 | - |
dc.subject.keywordPlus | HUMAN-CELLS | - |
dc.subject.keywordPlus | ENDONUCLEASE | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | RESOLVASE | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordAuthor | crystal structure | - |
dc.subject.keywordAuthor | flap DNA | - |
dc.subject.keywordAuthor | homologous recombination | - |
dc.subject.keywordAuthor | interstrand cross-link repair | - |
dc.subject.keywordAuthor | Mus81 | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
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