DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, MY | - |
dc.contributor.author | Kong, WH | - |
dc.contributor.author | Jung, HS | - |
dc.contributor.author | Hahn, SK | - |
dc.date.accessioned | 2016-03-31T07:32:54Z | - |
dc.date.available | 2016-03-31T07:32:54Z | - |
dc.date.created | 2015-02-12 | - |
dc.date.issued | 2014-04 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.other | 2014-OAK-0000031941 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/13751 | - |
dc.description.abstract | Despite extensive investigations on siRNA delivery systems for the past decade, there has been no clinically available product until now. In this work, reducible hyaluronic acid (HA)-siRNA conjugate was successfully synthesized and used to make a complex with cationic solid lipid nanoparticles (CSLNs) for the development of a liver specific siRNA delivery system. The reducible HA-siRNA conjugate was synthesized by the disulfide-thiol exchange reaction between pyridyldithiol modified HA and thiolated siRNA. The remaining pyridyldithiol was further blocked with cysteine. The biomimetic CSLNs were prepared by reconstituting the composition of natural apolipoprotein-free low density lipoproteins (LDLs). The formation of the HA-siRNA/CSLN complex was confirmed by gel electrophoresis (GE), dynamic light scattering (DLS), and atomic force microscopy (AFM). The HA-siRNA/CSLN complex showed remarkably low cytotoxicity and high transfection efficiency in the presence of serum. The therapeutic index (LC50/IC50) of the HAsiRNA/ CSLN complex was statistically much higher than that of a HA-siRNA conjugate or siRNA complexed with commercially available siRNA transfection reagents like in vivo jetPEI and INTERFERin, as well as an siRNA/CSLN complex. The HA-siRNA/CSLN complex can be effectively applied as a model system for the treatment of liver diseases, such as liver fibrosis and liver cancer. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.relation.isPartOf | RSC Advances | - |
dc.subject | IN-VIVO DELIVERY | - |
dc.subject | CELLULAR UPTAKE | - |
dc.subject | LIVER | - |
dc.subject | CELLS | - |
dc.subject | LUNG | - |
dc.subject | RNA | - |
dc.title | Hyaluronic acid–siRNA conjugates complexed with cationic solid lipid nanoparticles for target specific gene silencing | - |
dc.type | Article | - |
dc.contributor.college | 신소재공학과 | - |
dc.identifier.doi | 10.1039/C4RA01485E | - |
dc.author.google | Lee, MY | - |
dc.author.google | Kong, WH | - |
dc.author.google | Jung, HS | - |
dc.author.google | Hahn, SK | - |
dc.relation.volume | 4 | - |
dc.relation.issue | 37 | - |
dc.relation.startpage | 19338 | - |
dc.relation.lastpage | 19344 | - |
dc.contributor.id | 10149037 | - |
dc.relation.journal | RSC Advnaces | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | RSC Advances, v.4, no.37, pp.19338 - 19344 | - |
dc.identifier.wosid | 000335559100041 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 19344 | - |
dc.citation.number | 37 | - |
dc.citation.startPage | 19338 | - |
dc.citation.title | RSC Advances | - |
dc.citation.volume | 4 | - |
dc.contributor.affiliatedAuthor | Hahn, SK | - |
dc.identifier.scopusid | 2-s2.0-84899891421 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 6 | - |
dc.description.scptc | 4 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | IN-VIVO DELIVERY | - |
dc.subject.keywordPlus | CELLULAR UPTAKE | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | LUNG | - |
dc.subject.keywordPlus | RNA | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
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