DC Field | Value | Language |
---|---|---|
dc.contributor.author | Youngkyu Kim | - |
dc.contributor.author | Eung-Sam Kim | - |
dc.contributor.author | Yoonhee Lee | - |
dc.contributor.author | Joung-Hun Kim | - |
dc.contributor.author | Bong Chu Shim | - |
dc.contributor.author | Seong Moon Cho | - |
dc.contributor.author | Jeong Soo Lee | - |
dc.contributor.author | Park, JW | - |
dc.date.accessioned | 2016-03-31T07:59:58Z | - |
dc.date.available | 2016-03-31T07:59:58Z | - |
dc.date.created | 2014-10-01 | - |
dc.date.issued | 2014-10-01 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.other | 2014-OAK-0000030484 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/14335 | - |
dc.description.abstract | The importance of DNA sequencing in the life sciences and personalized medicine is continually increasing. Single-molecule sequencing methods have been developed to analyze DNA directly without the need for amplification. Here, we present a new approach to sequencing single DNA molecules using atomic force microscopy (AFM). In our approach, four surface conjugated nucleotides were examined sequentially with a DNA polymerase immobilized AFM tip. By observing the specific rupture events upon examination of a matching nucleotide, we could determine the template base bound in the polymerase's active site. The subsequent incorporation of the complementary base in solution enabled the next base to be read. Additionally, we observed that the DNA polymerase could incorporate the surface-conjugated dGTP when the applied force was controlled by employing the force-clamp mode. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.relation.isPartOf | Journal of the American Chemical Society | - |
dc.title | Reading Single DNA with DNA Polymerase Followed by Atomic Force Microscopy | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1021/JA5063983. | - |
dc.author.google | Kim, Y | - |
dc.author.google | Kim, ES | - |
dc.author.google | Lee, Y | - |
dc.author.google | Kim, JH | - |
dc.author.google | Shim, BC | - |
dc.author.google | Cho, SM | - |
dc.author.google | Lee, JS | - |
dc.author.google | Park, JW | - |
dc.relation.volume | 136 | - |
dc.relation.issue | 39 | - |
dc.relation.startpage | 13754 | - |
dc.relation.lastpage | 13760 | - |
dc.contributor.id | 10053210 | - |
dc.relation.journal | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Journal of the American Chemical Society, v.136, no.39, pp.13754 - 13760 | - |
dc.identifier.wosid | 000342608800052 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 13760 | - |
dc.citation.number | 39 | - |
dc.citation.startPage | 13754 | - |
dc.citation.title | Journal of the American Chemical Society | - |
dc.citation.volume | 136 | - |
dc.contributor.affiliatedAuthor | Joung-Hun Kim | - |
dc.contributor.affiliatedAuthor | Park, JW | - |
dc.identifier.scopusid | 2-s2.0-84907486679 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 14 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PHOSPHATE-LABELED NUCLEOTIDES | - |
dc.subject.keywordPlus | MOLECULE | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | NANOPORE | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | TECHNOLOGIES | - |
dc.subject.keywordPlus | PROCESSIVITY | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.