DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, YH | - |
dc.contributor.author | Lee, I | - |
dc.contributor.author | Choi, SH | - |
dc.contributor.author | Lee, OK | - |
dc.contributor.author | Shim, J | - |
dc.contributor.author | Lee, J | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Lee, EY | - |
dc.date.accessioned | 2016-03-31T08:12:59Z | - |
dc.date.available | 2016-03-31T08:12:59Z | - |
dc.date.created | 2014-03-06 | - |
dc.date.issued | 2013-05 | - |
dc.identifier.issn | 1381-1177 | - |
dc.identifier.other | 2013-OAK-0000029259 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/14815 | - |
dc.description.abstract | We developed magnetically-separable and highly-stable biocatalyst system of Mugil cephalus epoxide hydrolase (McEH) for enantioselective hydrolysis of racemic epoxides. McEH protein was adsorbed and cross-linked into mesoporous silica with bottle-neck mesopores, which effectively prevented the leaching of cross-linked McEH in a larger mesocellular pores. This ship-in-a-bottle approach allows for highly loaded and stable McEH system. For example, free McEH showed only 7.5% of initial activity under shaking condition at 80 h while ship-in-a-bottled McEH retained 79.5% of initial activity in the same condition. Stable McEH in magnetically-separable mesoporous silica could achieve the yield of 45% for the preparation of enantiopure (S)-styrene oxide with 98%ee. The magnetic nanoparticles pre-incorporated in mesoporous silica enabled an easy recovery of immobilized McEH for repetitive batch resolutions of racemic styrene oxide, allowing for more than 50% of the initial activity was retained after seven recycled uses. (C) 2012 Elsevier B.V. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC | - |
dc.subject | Epoxide hydrolase | - |
dc.subject | Mugil cephalus | - |
dc.subject | Nanoscale enzyme reactor | - |
dc.subject | Mesoporous silica | - |
dc.subject | Enzyme immobilization | - |
dc.subject | MUGIL-CEPHALUS | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | STABILIZATION | - |
dc.subject | NANOPARTICLES | - |
dc.subject | IMPROVEMENT | - |
dc.subject | CATALYSIS | - |
dc.subject | ENZYMES | - |
dc.subject | SYSTEM | - |
dc.title | Enhanced stability and reusability of marine epoxide hydrolase using ship-in-a-bottle approach with magnetically-separable mesoporous silica | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1016/J.MOLCATB.2012.12.012 | - |
dc.author.google | Kim, YH | - |
dc.author.google | Lee, I | - |
dc.author.google | Choi, SH | - |
dc.author.google | Lee, OK | - |
dc.author.google | Shim, J | - |
dc.author.google | Lee, J | - |
dc.author.google | Kim, J | - |
dc.author.google | Lee, EY | - |
dc.relation.volume | 89 | - |
dc.relation.startpage | 48 | - |
dc.relation.lastpage | 51 | - |
dc.contributor.id | 10138815 | - |
dc.relation.journal | JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, v.89, pp.48 - 51 | - |
dc.identifier.wosid | 000315552300008 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 51 | - |
dc.citation.startPage | 48 | - |
dc.citation.title | JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC | - |
dc.citation.volume | 89 | - |
dc.contributor.affiliatedAuthor | Lee, J | - |
dc.identifier.scopusid | 2-s2.0-84873045563 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 11 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | IMPROVEMENT | - |
dc.subject.keywordPlus | CATALYSIS | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordAuthor | Epoxide hydrolase | - |
dc.subject.keywordAuthor | Mugil cephalus | - |
dc.subject.keywordAuthor | Nanoscale enzyme reactor | - |
dc.subject.keywordAuthor | Mesoporous silica | - |
dc.subject.keywordAuthor | Enzyme immobilization | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
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