DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lim, JH | - |
dc.contributor.author | Seo, SW | - |
dc.contributor.author | Kim, SY | - |
dc.contributor.author | Jung, GY | - |
dc.date.accessioned | 2016-03-31T08:14:58Z | - |
dc.date.available | 2016-03-31T08:14:58Z | - |
dc.date.created | 2014-03-04 | - |
dc.date.issued | 2013-11 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.other | 2013-OAK-0000029104 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/14887 | - |
dc.description.abstract | The intracellular redox state plays an important role in the cellular physiology that determines the efficiency of chemical and biofuel production by microbial cell factories. However, it is difficult to achieve optimal redox rebalancing of synthetic pathways owing to the sensitive responses of cellular physiology according as the intracellular redox state changes. Here, we demonstrate optimal rebalancing of the intracellular redox state by model-driven control of expression using n-butanol production in Escherichia coli as a model system. The synthetic n-butanol production pathway was constructed by implementing synthetic constitutive promoters and designing synthetic 5'-untranslated regions (5'-UTR) for each gene. Redox rebalancing was achieved by anaerobically activating the pyruvate dehythogenase (PDH) complex and additionally tuning the expression level of NAD(+)-dependent formate dehydrogenase (fdh1 from Saccharomyces cerevisiae) through rational UTR engineering. Interestingly, efficient production of n-butanol required different amounts of reducing equivalents depending on whether the substrate was glucose or galactose. One intriguing implication of this work is that additional strain improvement can be achieved, even within given genetic components, through rebalancing intracellular redox state according to target products and substrates. (C) 2013 Elsevier Inc. All rights reserved | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | CADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | Metabolic Engineering | - |
dc.subject | Redox rebalancing | - |
dc.subject | Metabolic imbalance | - |
dc.subject | n-butanol | - |
dc.subject | Synthetic biology | - |
dc.subject | Metabolic engineering | - |
dc.subject | PYRUVATE-DEHYDROGENASE COMPLEX | - |
dc.subject | SYNTHETIC BIOLOGY | - |
dc.subject | FORMATE DEHYDROGENASE | - |
dc.subject | FATTY-ACIDS | - |
dc.subject | DESIGN | - |
dc.subject | EXPRESSION | - |
dc.subject | INITIATION | - |
dc.subject | GLUCOSE | - |
dc.subject | GENES | - |
dc.subject | K-12 | - |
dc.title | Model-driven rebalancing of the intracellular redox state for optimization of a heterologous n-butanol pathway in Escherichia coli. | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1016/J.YMBEN.2013.09.003 | - |
dc.author.google | Lim, JH | - |
dc.author.google | Seo, SW | - |
dc.author.google | Kim, SY | - |
dc.author.google | Jung, GY | - |
dc.relation.volume | 20 | - |
dc.relation.startpage | 56 | - |
dc.relation.lastpage | 62 | - |
dc.contributor.id | 10130678 | - |
dc.relation.journal | Metabolic Engineering | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Metabolic Engineering, v.20, pp.56 - 62 | - |
dc.identifier.wosid | 000328080100006 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 62 | - |
dc.citation.startPage | 56 | - |
dc.citation.title | Metabolic Engineering | - |
dc.citation.volume | 20 | - |
dc.contributor.affiliatedAuthor | Jung, GY | - |
dc.identifier.scopusid | 2-s2.0-84884927626 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 35 | - |
dc.description.scptc | 33 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PYRUVATE-DEHYDROGENASE COMPLEX | - |
dc.subject.keywordPlus | SYNTHETIC BIOLOGY | - |
dc.subject.keywordPlus | FORMATE DEHYDROGENASE | - |
dc.subject.keywordPlus | FATTY-ACIDS | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INITIATION | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | K-12 | - |
dc.subject.keywordAuthor | Redox rebalancing | - |
dc.subject.keywordAuthor | Metabolic imbalance | - |
dc.subject.keywordAuthor | n-butanol | - |
dc.subject.keywordAuthor | Synthetic biology | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
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