DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, K | - |
dc.contributor.author | Yang, JA | - |
dc.contributor.author | Lee, MY | - |
dc.contributor.author | Lee, H | - |
dc.contributor.author | Hahn, SK | - |
dc.date.accessioned | 2016-03-31T08:20:14Z | - |
dc.date.available | 2016-03-31T08:20:14Z | - |
dc.date.created | 2014-02-07 | - |
dc.date.issued | 2013-07 | - |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.other | 2013-OAK-0000028722 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/15079 | - |
dc.description.abstract | Despite wide applications of polymer-drug conjugates, there are only a few polymer-siRNA conjugates like poly(ethylene glycol) conjugated siRNA. In this work, reducible hyaluronic acid (HA)-siRNA conjugate was successfully developed for target specific systemic delivery of siRNA to the liver. The conjugation of siRNA to HA made it possible to form a compact nanocomplex of siRNA with relatively non-toxic linear polyethyleneimine (LPEI). After characterization of HA-siRNA conjugate by size exclusion chromatography (SEC) and gel electrophoresis, its complex formation with LPEI was investigated with a particle analyzer. The HA-siRNA/LPEI complex had a mean particle size of ca. 250 nm and a negative or neutral surface charge at physiological condition. The reducible HA-siRNA/LPEI complex showed a higher in vitro gene silencing efficiency than noncleavable HA-siRNA/LPEI complex. Furthermore, after systemic delivery, apolipoprotein B (ApoB) specific HA-siApoB/LPEI complex was target specifically delivered to the liver, which resulted in statistically significant reduction of ApoB mRNA expression in a dose dependent manner. The HA siRNA conjugate can be effectively applied as a model system to the treatment of liver diseases using various siRNAs and relatively nontoxic polycations. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.relation.isPartOf | BIOCONJUGATE CHEMISTRY | - |
dc.subject | IN-VIVO | - |
dc.subject | LINEAR POLYETHYLENIMINE | - |
dc.subject | INTRACELLULAR DELIVERY | - |
dc.subject | SYSTEMIC DELIVERY | - |
dc.subject | RNA INTERFERENCE | - |
dc.subject | COMPLEX | - |
dc.subject | DNA | - |
dc.subject | MICE | - |
dc.subject | THERAPEUTICS | - |
dc.subject | DERIVATIVES | - |
dc.title | Reducible Hyaluronic Acid-siRNA Conjugate for Target Specific Gene Silencing | - |
dc.type | Article | - |
dc.contributor.college | 신소재공학과 | - |
dc.identifier.doi | 10.1021/BC4001257 | - |
dc.author.google | Park, K | - |
dc.author.google | Yang, JA | - |
dc.author.google | Lee, MY | - |
dc.author.google | Lee, H | - |
dc.author.google | Hahn, SK | - |
dc.relation.volume | 24 | - |
dc.relation.issue | 7 | - |
dc.relation.startpage | 1201 | - |
dc.relation.lastpage | 1209 | - |
dc.contributor.id | 10149037 | - |
dc.relation.journal | BIOCONJUGATE CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCIE | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOCONJUGATE CHEMISTRY, v.24, no.7, pp.1201 - 1209 | - |
dc.identifier.wosid | 000322103200009 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 1209 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1201 | - |
dc.citation.title | BIOCONJUGATE CHEMISTRY | - |
dc.citation.volume | 24 | - |
dc.contributor.affiliatedAuthor | Hahn, SK | - |
dc.identifier.scopusid | 2-s2.0-84880387282 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 27 | - |
dc.description.scptc | 25 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | LINEAR POLYETHYLENIMINE | - |
dc.subject.keywordPlus | INTRACELLULAR DELIVERY | - |
dc.subject.keywordPlus | SYSTEMIC DELIVERY | - |
dc.subject.keywordPlus | RNA INTERFERENCE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | THERAPEUTICS | - |
dc.subject.keywordPlus | DERIVATIVES | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Organic | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
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