DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kyoung Je Cha | - |
dc.contributor.author | Jung Min Hong | - |
dc.contributor.author | Cho, DW | - |
dc.contributor.author | Kim, DS | - |
dc.date.accessioned | 2016-03-31T08:24:58Z | - |
dc.date.available | 2016-03-31T08:24:58Z | - |
dc.date.created | 2013-04-04 | - |
dc.date.issued | 2013-06 | - |
dc.identifier.issn | 1758-5082 | - |
dc.identifier.other | 2013-OAK-0000028331 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/15255 | - |
dc.description.abstract | During in vitro culture, cell fate and function, including cell adhesion, morphology, proliferation and differentiation, are affected by surface characteristics, such as geometry, wettability, hardness, chemistry and charge. This study replicated two different types of nanoengineered polystyrene surfaces (NPS) containing nanopillar (NPS-Pi) or nanopore (NPS-Po) arrays by hot embossing and investigated their topographical effects on cell behavior using osteoblast-like MC3T3-E1 cells. To mass-replicate NPS, rigid metal nano-stamps were manufactured by nickel electroforming onto two different nano-templates: (1) a nanopore-arrayed anodic aluminum oxide nano-template using two-step electrochemical oxidation and (2) a nanopillar-arrayed polymer using hot embossing process. The physical and mechanical properties of the NPS, including geometry, wettability, hardness and elastic modulus, were evaluated with the help of field emission-scanning electron microscopy, a contact angle meter, and a nanoindenter. The nanotopography maintained the bulk property, while drastically changing the surface properties. In vitro the NPS had significant effects on MC3T3-E1 cell morphology, attachment, proliferation and osteogenic differentiation compared to a flat substrate due to the altered physical and mechanical surface properties of the nanoengineered surface. Interestingly, the NPS-Po was more effective at enhancing cell proliferation and osteogenesis differentiation. One potential explanation for these results may be that the subcellular binding sites induced by the nanostructures changed the cell morphology and promoted contractile cytoskeletons, thereby enhancing osteogenic differentiation. This, which allows for the cost-effective replication of NPS and the control of cell behavior, has various applications with respect to biomedical and cell surface interaction studies, in addition to enhanced osteogenic cell fate and function. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | Institute of Physics | - |
dc.relation.isPartOf | Biofabrication | - |
dc.subject | OSTEOBLAST-LIKE CELLS | - |
dc.subject | STEM-CELLS | - |
dc.subject | ADHESION | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | NANOSCALE | - |
dc.subject | LITHOGRAPHY | - |
dc.subject | TOPOGRAPHY | - |
dc.subject | MORPHOLOGY | - |
dc.subject | SUBSTRATE | - |
dc.subject | MICRO | - |
dc.title | Enhanced osteogenic fate and function of MC3T3-E1 cells on nanoengineered polystyrene surfaces with nanopillar and nanopore arrays | - |
dc.type | Article | - |
dc.contributor.college | 기계공학과 | - |
dc.identifier.doi | 10.1088/1758-5082/5/2/025007 | - |
dc.author.google | Cha, KJ | - |
dc.author.google | Hong, JM | - |
dc.author.google | Cho, DW | - |
dc.author.google | Kim, DS | - |
dc.relation.volume | 5 | - |
dc.relation.issue | 2 | - |
dc.contributor.id | 10102903 | - |
dc.relation.journal | BIOFABRICATION | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Biofabrication, v.5, no.2, pp.25007 | - |
dc.identifier.wosid | 000318149900008 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 25007 | - |
dc.citation.title | Biofabrication | - |
dc.citation.volume | 5 | - |
dc.contributor.affiliatedAuthor | Cho, DW | - |
dc.contributor.affiliatedAuthor | Kim, DS | - |
dc.identifier.scopusid | 2-s2.0-84877742211 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 23 | - |
dc.description.scptc | 22 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | NANOSCALE | - |
dc.subject.keywordPlus | LITHOGRAPHY | - |
dc.subject.keywordPlus | MORPHOLOGY | - |
dc.subject.keywordPlus | MICRO | - |
dc.subject.keywordPlus | NANOFABRICATION | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | TOPOGRAPHY | - |
dc.subject.keywordPlus | ROUGHNESS | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.