Development of a bone reconstruction technique using a solid free-form fabrication (SFF)-based drug releasing scaffold and adipose-derived stem cells
SCIE
SCOPUS
- Title
- Development of a bone reconstruction technique using a solid free-form fabrication (SFF)-based drug releasing scaffold and adipose-derived stem cells
- Authors
- Lee, JW; Kim, KJ; Kang, KS; Chen, SC; Rhie, JW; Cho, DW
- Date Issued
- 2013-07
- Publisher
- WILEY-BLACKWELL
- Abstract
- For tissue regeneration, three essential components of scaffolds, signals (biomolecules), and cells are required. Moreover, because bony defects are three-dimensional in many clinical circumstances, an exact 3D scaffold is important. Therefore, we proposed an effective reconstruction tool for cranial defects using human adipose-derived stem cells (hADSCs) and a 3D functional scaffold fabricated by solid free-form fabrication (SFF) technology that secretes biomolecules. We fabricated poly(propylene fumarate)-based 3D scaffolds with embedded microsphere-deliverable bone morphogenetic protein-2 (BMP-2) by microstereolithography. BMP-2-loaded SFF scaffolds with/without hADSCs (SFF/BMP/hADSCs scaffolds and SFF/BMP scaffolds, respectively) and BMP-2-unloaded SFF scaffolds (SFF scaffolds) were then implanted in rat crania, and in vivo bone formation was observed. Analyses of bone formation areas using micro-computed tomography (micro-CT) showed the superiority of SFF/BMP/hADSCs scaffolds. Hematoxylin and eosin stain, Masson's trichrome stain, and collagen type-I stain supported the results of the micro-CT scan. And human leukocyte antigen-ABC showed that seeded, differentiated hADSCs were well grown and changed to the bone tissue at the inside of the scaffold. Results showed that our combination of a functional 3D scaffold and hADSCs may be a useful tool for improving the reconstruction quality of severe bony defects in which thick bone is required. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
- Keywords
- bone-tissue engineering; scaffold; bone morphogenetic protein (BMP); human adipose-derived stem cells (hADSCs); solid free-form fabrication (SFF); GROWTH-FACTOR; GENE-EXPRESSION; CANINE MODEL; IN-VITRO; TISSUE; DIFFERENTIATION; REGENERATION; BMP-2; MICROSTEREOLITHOGRAPHY; POLYMER
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/15260
- DOI
- 10.1002/JBM.A.34485
- ISSN
- 1549-3296
- Article Type
- Article
- Citation
- JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, vol. 101A, no. 7, page. 1865 - 1875, 2013-07
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.