DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jong Moon Park | - |
dc.contributor.author | Kim, YM | - |
dc.contributor.author | Min, BE | - |
dc.contributor.author | Jung, GY | - |
dc.contributor.author | Park, JM | - |
dc.date.accessioned | 2016-03-31T08:34:00Z | - |
dc.date.available | 2016-03-31T08:34:00Z | - |
dc.date.created | 2012-01-16 | - |
dc.date.issued | 2011-08 | - |
dc.identifier.issn | 1555-130X | - |
dc.identifier.other | 2011-OAK-0000027430 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/15593 | - |
dc.description.abstract | The NAD(P)H- or ferredoxin-dependent hydrogen production pathway is known to have a huge potential for improving its production yield compared to the formate hydrogen lyase (FHL) route. To improve the hydrogen production yield through this pathway, rebalancing the NADPH/NADH ratio based on the cofactor preference is important, in addition to enhancing the NAD(P)H pool by reducing the competing pathways. NADPH:ferredoxin oxidoreductase coupled with [FeFe]-hydrogenase preferably utilizes NADPH as an electron donor. Therefore, directing NADPH should be required to improve the hydrogen production yield. For balancing the NADPH/NADH ratio, nicotinamide nucleotide transhydrogenase has a key role, and Escherichia coli has the two transhydrogenases, PntAB and SthA. In this study, several recombinants were constructed to up-regulate pntAB or to down-regulate sthA in the competing-/dhA-deleted E. coli expressing [FeFe]-hydrogenase system. As a result, an about 3.9-fold increased hydrogen yield was observed in the pntAB-overexpressed and sthA-deleted strain. Therefore, the combination of IdhA down-regulation and NADPH/NADH ratio rebalancing via transhydrogenase engineering can improve the hydrogen production yield in the NADPH- or ferredoxin-dependent hydrogen production pathway. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | American Scientific Publishers | - |
dc.relation.isPartOf | JOURNAL OF NANOELECTRONICS AND OPTOELECTRONICS | - |
dc.title | Improvement of Hydrogen Production Yield by Rebalancing NADPH/NADH Ratio in a Recombinant Escherichia coil | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1166/JNO.2011.1182 | - |
dc.author.google | Cho, HS | - |
dc.author.google | Kim, YM | - |
dc.author.google | Min, BE | - |
dc.author.google | Jung, GY | - |
dc.author.google | Park, JM | - |
dc.relation.volume | 6 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 343 | - |
dc.relation.lastpage | 347 | - |
dc.contributor.id | 10130678 | - |
dc.relation.journal | Journal of Nanoelectronics and Optoelectronics | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF NANOELECTRONICS AND OPTOELECTRONICS, v.6, no.3, pp.343 - 347 | - |
dc.identifier.wosid | 000299583000028 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 347 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 343 | - |
dc.citation.title | JOURNAL OF NANOELECTRONICS AND OPTOELECTRONICS | - |
dc.citation.volume | 6 | - |
dc.contributor.affiliatedAuthor | Jung, GY | - |
dc.contributor.affiliatedAuthor | Park, JM | - |
dc.identifier.scopusid | 2-s2.0-84863134487 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 3 | - |
dc.description.scptc | 4 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article; Proceedings Paper | - |
dc.subject.keywordPlus | COLI | - |
dc.subject.keywordPlus | TRANSHYDROGENASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | PNTAB | - |
dc.subject.keywordAuthor | NADPH | - |
dc.subject.keywordAuthor | Biological Hydrogen Production | - |
dc.subject.keywordAuthor | Transhydrogenase | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.relation.journalWebOfScienceCategory | Engineering, Electrical & Electronic | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Physics | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.