Aberration compensation for objective phase curvature in phase holographic microscopy

Abstract

In this study, we proposed a simple and fast numerical approach to compensate for aberrations induced by objective phase curvature. This method is based on the extraction of virtual background phase from reconstructed phase values using a line profile, followed by subtraction of the virtual background phase from the reconstructed phase image. The performance and feasibility of the method were demonstrated by applying it to the phase imaging of polystyrene microspheres and red blood cells. (C) 2012 Optical Society of America