DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, CS | - |
dc.contributor.author | Seo, JH | - |
dc.contributor.author | Cha, HJ | - |
dc.date.accessioned | 2016-03-31T08:38:18Z | - |
dc.date.available | 2016-03-31T08:38:18Z | - |
dc.date.created | 2013-03-26 | - |
dc.date.issued | 2012-08-07 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.other | 2012-OAK-0000027207 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/15713 | - |
dc.description.abstract | The development of analytical tools is important for understanding the infection mechanisms of pathogenic bacteria or viruses. In the present work, a functional carbohydrate microarray combined with a fluorescence immunoassay was developed to analyze the interactions of Vibrio cholerae toxin (ctx) proteins and GM1-related carbohydrates. Ctx proteins were loaded onto the surface-immobilized GM1 pentasaccharide and six related carbohydrates, and their binding affinities were detected immunologically. The analysis of the ctx-carbohydrate interactions revealed that the intrinsic selectivity of do( was GM1 pentasaccharide >> GM2 tetrasaccharide > asialo >= GM1 tetrasaccharide >= GM3trisaccharide, indicating that a two-finger grip formation and the terminal monosaccharides play important roles in the ctx-GM1 interaction. In addition, whole cholera toxin (ctxAB(5)) had a stricter substrate specificity and a stronger binding affinity than only the cholera toxin B subunit (ctxB). On the basis of the quantitative analysis, the carbohydrate microarray showed the sensitivity of detection of the ctxAB(5)-GM1 interaction with a limit-of-detection (LOD) of 2 ng mL(-1) (23 pM), which is comparable to other reported high sensitivity assay tools. In addition, the carbohydrate microarray successfully detected the actual toxin directly secreted from V. cholerae, without showing cross-reactivity to other bacteria. Collectively, these results demonstrate that the functional carbohydrate microarray is suitable for analyzing toxin protein-carbohydrate interactions and can be applied as a biosensor for toxin detection. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ACS Publications | - |
dc.relation.isPartOf | Analytical Chemistry | - |
dc.subject | SURFACE-PLASMON RESONANCE | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | LIGAND-BINDING | - |
dc.subject | FLOW-CYTOMETRY | - |
dc.subject | ENTERO-TOXIN | - |
dc.subject | PROTEIN | - |
dc.subject | GM1 | - |
dc.subject | OLIGOSACCHARIDE | - |
dc.subject | SPECIFICITY | - |
dc.subject | RECEPTOR | - |
dc.title | Functional Interaction Analysis of GM1-Related Carbohydrates and Vibrio cholerae Toxins Using Carbohydrate Microarray | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1021/AC301511T | - |
dc.author.google | Kim, CS | - |
dc.author.google | Seo, JH | - |
dc.author.google | Cha, HJ | - |
dc.relation.volume | 84 | - |
dc.relation.issue | 15 | - |
dc.relation.startpage | 6884 | - |
dc.relation.lastpage | 6890 | - |
dc.contributor.id | 10057405 | - |
dc.relation.journal | Analytical Chemistry | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Analytical Chemistry, v.84, no.15, pp.6884 - 6890 | - |
dc.identifier.wosid | 000307159200087 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 6890 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 6884 | - |
dc.citation.title | Analytical Chemistry | - |
dc.citation.volume | 84 | - |
dc.contributor.affiliatedAuthor | Cha, HJ | - |
dc.identifier.scopusid | 2-s2.0-84864592892 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 15 | - |
dc.description.scptc | 14 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | HEAT-LABILE ENTEROTOXIN | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | LIGAND-BINDING | - |
dc.subject.keywordPlus | GM1 | - |
dc.subject.keywordPlus | OLIGOSACCHARIDE | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | THERMODYNAMICS | - |
dc.subject.keywordPlus | GANGLIOSIDES | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
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