DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, CS | - |
dc.contributor.author | Bong-Hyuk Choi | - |
dc.contributor.author | Jeong Hyun Seo | - |
dc.contributor.author | Lim, G | - |
dc.contributor.author | Hyung Joon Cha | - |
dc.date.accessioned | 2016-03-31T08:38:26Z | - |
dc.date.available | 2016-03-31T08:38:26Z | - |
dc.date.created | 2013-03-06 | - |
dc.date.issued | 2013-03-15 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.other | 2013-OAK-0000027202 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/15718 | - |
dc.description.abstract | A whole cell array biosensor for the efficient detection of neurotoxic organophosphate compounds (OPs) was developed through the immobilization of recombinant Escherichia coli cells containing periplasmic-expressing organophosphorus hydrolase (OPH) onto the surface of a 96-well microplate using mussel adhesive protein (MAP) as a microbial cell-immobilizing linker. Both the paraoxon-hydrolyzing activity and fluorescence microscopy analyses demonstrated that the use of MAP in a whole cell biosensor increased the cell-immobilizing efficiency and enhanced the stability of immobilized cells compared to a simple physical adsorption-based whole cell system. Scanning electron microscopic analyses also showed that the E. coli cells were effectively immobilized on the MAP-coated surface without any pretreatment steps. The whole cell array biosensor system, prepared using optimal MAP coating (50 mu g/cm(2)) and cell loading (4 OD600), detected paraoxon levels as low as 5 mu M with high reproducibility, and its quantitative detection range was similar to 5-320 mu M. The MAP-based whole cell array biosensor showed a good long-term stability for 28 day with 80% retained activity and a reusability of up to 20 times. In addition, paraoxon in tap water was also successfully detected without a reduction in sensitivity. Our results indicate that the proposed MAP-based whole cell array system could be used as a potential platform for a stable and reusable whole cell biosensor. (C) 2012 Elsevier B.V. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | Biosensors and Bioelectronics | - |
dc.subject | Whole cell array biosensor | - |
dc.subject | Mussel adhesive protein | - |
dc.subject | Cell-immobilizing linker | - |
dc.subject | Organophosphorus hydrolase | - |
dc.subject | Escherichia coli | - |
dc.subject | RECOMBINANT ESCHERICHIA-COLI | - |
dc.subject | AMPEROMETRIC MICROBIAL BIOSENSOR | - |
dc.subject | NERVE AGENTS | - |
dc.subject | METHYL PARATHION | - |
dc.subject | PERIPLASMIC SECRETION | - |
dc.subject | MYTILUS-EDULIS | - |
dc.subject | HYDROLASE | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | ELECTRODE | - |
dc.subject | PESTICIDES | - |
dc.title | Mussel adhesive protein-based whole cell array biosensor for detection of organophosphorus compounds | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1016/J.BIOS.2012.08.022 | - |
dc.author.google | Kim, CS | - |
dc.author.google | Choi, BH | - |
dc.author.google | Seo, JH | - |
dc.author.google | Lim, G | - |
dc.author.google | Cha, HJ | - |
dc.relation.volume | 41 | - |
dc.relation.issue | 15 | - |
dc.relation.startpage | 199 | - |
dc.relation.lastpage | 204 | - |
dc.contributor.id | 10057405 | - |
dc.relation.journal | Biosensors and Bioelectronics | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Biosensors and Bioelectronics, v.41, pp.199 - 204 | - |
dc.identifier.wosid | 000314191300027 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 204 | - |
dc.citation.startPage | 199 | - |
dc.citation.title | Biosensors and Bioelectronics | - |
dc.citation.volume | 41 | - |
dc.contributor.affiliatedAuthor | Lim, G | - |
dc.contributor.affiliatedAuthor | Hyung Joon Cha | - |
dc.identifier.scopusid | 2-s2.0-84870774652 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 20 | - |
dc.description.scptc | 23 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RECOMBINANT ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | AMPEROMETRIC MICROBIAL BIOSENSOR | - |
dc.subject.keywordPlus | NERVE AGENTS | - |
dc.subject.keywordPlus | METHYL PARATHION | - |
dc.subject.keywordPlus | PERIPLASMIC SECRETION | - |
dc.subject.keywordPlus | MYTILUS-EDULIS | - |
dc.subject.keywordPlus | HYDROLASE | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | ELECTRODE | - |
dc.subject.keywordPlus | PESTICIDES | - |
dc.subject.keywordAuthor | Whole cell array biosensor | - |
dc.subject.keywordAuthor | Mussel adhesive protein | - |
dc.subject.keywordAuthor | Cell-immobilizing linker | - |
dc.subject.keywordAuthor | Organophosphorus hydrolase | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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