DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, EungSam | - |
dc.contributor.author | Kim, JungSook | - |
dc.contributor.author | Lee, Yoonhee | - |
dc.contributor.author | Choi, KY | - |
dc.contributor.author | Park, JW | - |
dc.date.accessioned | 2016-03-31T08:39:35Z | - |
dc.date.available | 2016-03-31T08:39:35Z | - |
dc.date.created | 2013-03-20 | - |
dc.date.issued | 2012-07 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.other | 2012-OAK-0000027130 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/15762 | - |
dc.description.abstract | Nick-sealing of a single DNA duplex was studied with the use of atomic force microscopy (AFM). To form a nick between a 47mer DNA and a 24mer DNA, the complementary 71mer template DNA immobilized on an AFM tip was hybridized with the 47mer DNA and brought into contact with the 24mer DNA on a substrate surface. The AFM tip and substrate surface were modified with dendron molecules to ensure the formation of a single DNA duplex. When a single nick in the DNA duplex was sealed by DNA ligase during a pause, an increase in the unbinding force was observed after the pause. The change from 24.0 +/- 4.4 piconewtons (pN) to 62.8 +/- 14.6 pN matched well with the resulting DNA length (71 bp). Additionally, a 30 s pause showed a 3-fold higher nick-sealing probability (60%) than a 10 s pause, while the probability did not increase with a 120 s pause. In the presence of free 47mer DNAs in solution, the single nick-sealing event could be repeated at other positions. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.relation.isPartOf | ACS Nano | - |
dc.subject | DNA ligation | - |
dc.subject | single-molecule manipulation | - |
dc.subject | atomic force microscopy | - |
dc.subject | unbinding force measurement | - |
dc.subject | dendron-modified surface | - |
dc.subject | DIP-PEN NANOLITHOGRAPHY | - |
dc.subject | ENZYMATIC NANOLITHOGRAPHY | - |
dc.subject | PROTEIN | - |
dc.subject | MOLECULES | - |
dc.subject | LIGASE | - |
dc.subject | SPECTROSCOPY | - |
dc.subject | GOLD | - |
dc.title | Following the DNA Ligation of a Single Duplex Using Atomic Force Microscopy | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.1021/NN301200K | - |
dc.author.google | Kim, ES | - |
dc.author.google | Kim, JS | - |
dc.author.google | Lee, Y | - |
dc.author.google | Choi, KY | - |
dc.author.google | Park, JW | - |
dc.relation.volume | 6 | - |
dc.relation.issue | 7 | - |
dc.relation.startpage | 6108 | - |
dc.relation.lastpage | 6114 | - |
dc.contributor.id | 10052985 | - |
dc.relation.journal | ACS Nano | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ACS Nano, v.6, no.7, pp.6108 - 6114 | - |
dc.identifier.wosid | 000306673800041 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 6114 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 6108 | - |
dc.citation.title | ACS Nano | - |
dc.citation.volume | 6 | - |
dc.contributor.affiliatedAuthor | Choi, KY | - |
dc.contributor.affiliatedAuthor | Park, JW | - |
dc.identifier.scopusid | 2-s2.0-84864195068 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 10 | - |
dc.description.scptc | 9 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | DIP-PEN NANOLITHOGRAPHY | - |
dc.subject.keywordPlus | ENZYMATIC NANOLITHOGRAPHY | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | MOLECULES | - |
dc.subject.keywordPlus | LIGASE | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordAuthor | DNA ligation | - |
dc.subject.keywordAuthor | single-molecule manipulation | - |
dc.subject.keywordAuthor | atomic force microscopy | - |
dc.subject.keywordAuthor | unbinding force measurement | - |
dc.subject.keywordAuthor | dendron-modified surface | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
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