Secretory pathway research: the more experimental systems the better
SCIE
SCOPUS
- Title
- Secretory pathway research: the more experimental systems the better
- Authors
- Jurgen Denecke; Fernando Aniento; Lorenzo Frigerio; Chris Hawes; Hwang, I; Jaideep Mathur; Jean-Marc Neuhaus; David G. Robinson
- Date Issued
- 2012-04
- Publisher
- ASPB
- Abstract
- Transient gene expression, in plant protoplasts or specific plant tissues, is a key technique in plant molecular cell biology, aimed at exploring gene products and their modifications to examine functional subdomains, their interactions with other biomolecules, and their subcellular localization. Here, we highlight some of the major advantages and potential pitfalls of the most commonly used transient gene expression models and illustrate how ectopic expression and the use of dominant mutants can provide insights into protein function.
- Keywords
- VACUOLAR SORTING RECEPTOR; ARABIDOPSIS MESOPHYLL PROTOPLASTS; GOLGI NETWORK/EARLY ENDOSOME; ENDOPLASMIC-RETICULUM STRESS; DIRECT GENE-TRANSFER; IN-VIVO ANALYSIS; PLANT-CELLS; PREVACUOLAR COMPARTMENT; TRANSIENT EXPRESSION; TOBACCO PROTOPLASTS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/15869
- DOI
- 10.1105/TPC.112.096362
- ISSN
- 1040-4651
- Article Type
- Article
- Citation
- Plant cell, vol. 24, no. 4, page. 1316 - 1326, 2012-04
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- There are no files associated with this item.
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