DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gwak, J | - |
dc.contributor.author | Hwang, SG | - |
dc.contributor.author | Park, HS | - |
dc.contributor.author | Choi, SR | - |
dc.contributor.author | Park, SH | - |
dc.contributor.author | Kim, H | - |
dc.contributor.author | Ha, NC | - |
dc.contributor.author | Bae, SJ | - |
dc.contributor.author | Han, JK | - |
dc.contributor.author | Kim, DE | - |
dc.contributor.author | Cho, JW | - |
dc.contributor.author | Oh, S | - |
dc.date.accessioned | 2016-03-31T08:45:22Z | - |
dc.date.available | 2016-03-31T08:45:22Z | - |
dc.date.created | 2013-03-05 | - |
dc.date.issued | 2012-01 | - |
dc.identifier.issn | 1001-0602 | - |
dc.identifier.other | 2012-OAK-0000026676 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/15978 | - |
dc.description.abstract | The Wnt/beta-catenin pathway plays important roles in the differentiation of multiple cell types, including mesenchymal stem cells. Using a cell-based chemical screening assay with a synthetic chemical library of 270 000 compounds, we identified the compound SKL2001 as a novel agonist of the Wnt/beta-catenin pathway and uncovered its molecular mechanism of action. SKL2001 upregulated beta-catenin responsive transcription by increasing the intracellular beta-catenin protein level and inhibited the phosphorylation of beta-catenin at residues Ser33/37/Thr41 and Ser45, which would mark it for proteasomal degradation, without affecting CK1 and GSK-3 beta enzyme activities. Biochemical analysis revealed that SKL2001 disrupted the Axin/beta-catenin interaction, which is a critical step for CK1- and GSK-3 beta-mediated phosphorylation of beta-catenin at Ser33/37/Thr41 and Ser45. The treatment of mesenchymal stem cells with SKL2001 promoted osteoblastogenesis and suppressed adipocyte differentiation, both of which were accompanied by the activation of Wnt/beta-catenin pathway. Our findings provide a new strategy to regulate mesenchymal stem cell differentiation by modulation of the Wnt/beta-catenin pathway. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | INST BIOCHEMISTRY & CELL BIOLOGY | - |
dc.relation.isPartOf | CELL RESEARCH | - |
dc.subject | Wnt pathway | - |
dc.subject | Axin/beta-catenin complex | - |
dc.subject | small molecule | - |
dc.subject | mesenchymal stem cell | - |
dc.subject | BETA-CATENIN | - |
dc.subject | DESTRUCTION COMPLEX | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | BONE MASS | - |
dc.subject | IN-VITRO | - |
dc.subject | KAPPA-B | - |
dc.subject | ADIPOGENESIS | - |
dc.subject | OSTEOBLAST | - |
dc.subject | PATHWAY | - |
dc.subject | INHIBITORS | - |
dc.title | Small molecule-based disruption of the Axin/beta-catenin protein complex regulates mesenchymal stem cell differentiation | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1038/CR.2011.127 | - |
dc.author.google | Gwak, J | - |
dc.author.google | Hwang, SG | - |
dc.author.google | Park, HS | - |
dc.author.google | Choi, SR | - |
dc.author.google | Park, SH | - |
dc.author.google | Kim, H | - |
dc.author.google | Ha, NC | - |
dc.author.google | Bae, SJ | - |
dc.author.google | Han, JK | - |
dc.author.google | Kim, DE | - |
dc.author.google | Cho, JW | - |
dc.author.google | Oh, S | - |
dc.relation.volume | 22 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 237 | - |
dc.relation.lastpage | 247 | - |
dc.contributor.id | 10138853 | - |
dc.relation.journal | CELL RESEARCH | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | CELL RESEARCH, v.22, no.1, pp.237 - 247 | - |
dc.identifier.wosid | 000299312900022 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 247 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 237 | - |
dc.citation.title | CELL RESEARCH | - |
dc.citation.volume | 22 | - |
dc.contributor.affiliatedAuthor | Han, JK | - |
dc.identifier.scopusid | 2-s2.0-84855497795 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 44 | - |
dc.description.scptc | 39 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | OSTEOBLAST | - |
dc.subject.keywordPlus | ADIPOGENESIS | - |
dc.subject.keywordPlus | WNT10B | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordPlus | PHOSPHORYLATION/DEGRADATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordAuthor | Wnt pathway | - |
dc.subject.keywordAuthor | Axin/beta-catenin complex | - |
dc.subject.keywordAuthor | small molecule | - |
dc.subject.keywordAuthor | mesenchymal stem cell | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
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