A highly sensitive aptasensor towards Plasmodium lactate dehydrogenase for the diagnosis of malaria
SCIE
SCOPUS
- Title
- A highly sensitive aptasensor towards Plasmodium lactate dehydrogenase for the diagnosis of malaria
- Authors
- Seonghwan Lee; Kyung-Mi Song; Weejeong Jeon; Hunho Jo; Yoon-Bo Shim; Ban, C
- Date Issued
- 2012-05-15
- Publisher
- elsevier
- Abstract
- Finding a highly sensitive diagnostic technique for malaria has challenged scientists for the last century. In the present study, we identified versatile single-strand DNA aptamers for Plasmodium lactate dehydrogenase (pLDH), a biomarker for malaria, via the Systematic Evolution of Ligands by EXponential enrichment (SELEX). The pLDH aptamers selectively bound to the target proteins with high sensitivity (K-d = 16.8-49.6 nM). The selected aptamers were characterized using an electrophoretic mobility shift assay, a quartz crystal microbalance, a fluorescence assay, and circular dichroism spectroscopy. We also designed a simple aptasensor using electrochemical impedance spectroscopy; both Plasmodium vivax LDH and Plasmodium falciparum LDH were selectively detected with a detection limit of 1 pM. Furthermore, the pLDH aptasensor clearly distinguished between malaria-positive blood samples of two major species (P. vivax and P. falciparum) and a negative control, indicating that it may be a useful tool for the diagnosis, monitoring, and surveillance of malaria. (C) 2012 Elsevier B.V. All rights reserved.
- Keywords
- Aptamer; SELEX; Diagnosis; Malaria; Plasmodium lactate dehydrogenase; Impedance spectroscopy; ELECTROCHEMICAL DETECTION; EXPONENTIAL ENRICHMENT; SYSTEMATIC EVOLUTION; MAGNETIC BEADS; DNA; APTAMER; LIGANDS; RNA; HYBRIDIZATION; PARASITES
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16233
- DOI
- 10.1016/J.BIOS.2012.03.003
- ISSN
- 0956-5663
- Article Type
- Article
- Citation
- BIOSENSORS & BIOELECTRONICS, vol. 35, no. 1, page. 291 - 296, 2012-05-15
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