The crystal structure of flap endonuclease-1 from Methanococcus jannaschii
SCIE
SCOPUS
- Title
- The crystal structure of flap endonuclease-1 from Methanococcus jannaschii
- Authors
- Hwang, KY; Baek, K; Kim, HY; Cho, Y
- Date Issued
- 1998-08
- Publisher
- Nature Publishing Group
- Abstract
- Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 Angstrom resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.
- Keywords
- COLI DNA-POLYMERASE; EXONUCLEASE; REPLICATION; REPAIR; 5' -EXONUCLEASE; CLEAVAGE; SEQUENCE; HOMOLOGY; DOMAIN; FEN-1
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16308
- DOI
- 10.1038/1406
- ISSN
- 1072-8368
- Article Type
- Article
- Citation
- NATURE STRUCTURAL BIOLOGY, vol. 5, no. 8, page. 707 - 713, 1998-08
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- There are no files associated with this item.
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