Proteomic Analysis of Microvesicles Derived from Human Mesenchymal Stem Cells
SCIE
SCOPUS
- Title
- Proteomic Analysis of Microvesicles Derived from Human Mesenchymal Stem Cells
- Authors
- Kim, HS; Choi, DY; Yun, SJ; Choi, SM; Kang, JW; Jung, JW; Hwang, D; Kim, KP; Kim, DW
- Date Issued
- 2012-02
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Mesenchymal stem cells (MSCs) have emerged as a promising means for treating degenerative or incurable diseases. Recent studies have shown that microvesicles (MVs) from MSCs (MSC-MVs) contribute to recovery of damaged tissues in animal disease models. Here, we profiled the MSC-MV proteome to investigate their therapeutic effects. LC-MS/MS analysis of MSC-MVs identified 730 MV proteins. The MSC-MV proteome included five positive and two variable known markers of MSCs, but no negative marker, as well as 43 surface receptors and signaling molecules controlling self-renewal and differentiation of MSCs. Functional enrichment analysis showed that cellular processes represented by the MSC-MV proteins include cell proliferation, adhesion, migration, and morphogenesis. Integration of MSC's self-renewal and differentiation-related genes and the proteome of MSC-conditioned media (MSC-CM) with the MSC-MV proteome revealed potential MV protein candidates that can be associated with the therapeutic effects of MSC-MVs: (1) surface receptors (PDGFRB, EGFR, and PLAUR); (2) signaling molecules (RRAS/NRAS, MAPK1, GNA13/GNG12, CDC42, and VAV2); (3) cell adhesion (FN1, EZR, IQGAP1, CD47, integrins, and LGALS1/LGALS3); and (4) MSC-associated antigens (CD9, CD63, CD81, CD109, CD151, CD248, and CD276). Therefore, the MSC-MV proteome provides a comprehensive basis for understanding the potential of MSC-MVs to affect tissue repair and regeneration.
- Keywords
- mesenchymal stem cells; microvesicle; proteomics; self-renewal; tissue regeneration; STROMAL CELLS; GROWTH-FACTOR; EPITHELIAL-CELLS; PROGENITOR CELLS; SELF-RENEWAL; MARROW; DIFFERENTIATION; CANCER; PROLIFERATION; REGENERATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16375
- DOI
- 10.1021/PR200682Z
- ISSN
- 1535-3893
- Article Type
- Article
- Citation
- JOURNAL OF PROTEOME RESEARCH, vol. 11, no. 2, page. 839 - 849, 2012-02
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.