DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, EungSam | - |
dc.contributor.author | Shim, ChangKyoon | - |
dc.contributor.author | Lee, Jae W | - |
dc.contributor.author | Park, JW | - |
dc.contributor.author | Choi, KY | - |
dc.date.accessioned | 2016-03-31T08:59:33Z | - |
dc.date.available | 2016-03-31T08:59:33Z | - |
dc.date.created | 2013-03-08 | - |
dc.date.issued | 2012-04 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.other | 2012-OAK-0000025622 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/16437 | - |
dc.description.abstract | The proper orientation and lateral spacing of antibody molecules are a crucial element for an on-chip immunoassay in which the antibody or its antigen-binding fragments are immobilized on a solid surface. We covalently immobilized a modified protein G (Cys-protein G: protein G with only an N-terminal cysteine) on a dendron-coated surface to control its orientation and lateral spacing simultaneously. The cysteine-specific immobilization of Cys-protein G through the N-terminal cysteine resulted in 2.2-fold higher binding efficiency of Cys-protein G to IgG(2a) capture antibody than its random immobilization via lysine residues. The lateral spacing of 3.2 nm due to the surface modification with the 9-acid dendron molecule contributed to a 1.5-fold increase in the antibody-binding ability of Cys-protein G. Topographic images of atomic force microscopy exhibited a uniform coverage of Cys-protein G molecules immobilized on the thiol-reactive 9-acid dendron surface and homogeneous distribution of antibody bound to Cys-protein G. In the sandwich immunoassay, the control of the orientation of Cys-protein G led to 10-fold higher detection capability for rIL-2 compared with the randomly oriented protein G. The synergistic advantage of the unidirectional orientation and homogeneous lateral spacing of Cys-protein Gs on the dendron-coated surface can be applied to the development of more sensitive and reproducible antibody microarrays. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | Royal Society of Chemistry | - |
dc.relation.isPartOf | Analyst | - |
dc.title | Synergistic effect of orientation and lateral spacing of protein G on an on-chip immunoassay. | - |
dc.type | Article | - |
dc.contributor.college | 융합생명공학부 | - |
dc.identifier.doi | 10.1039/C2AN16137K | - |
dc.author.google | Kim, ES | - |
dc.author.google | Shim, CK | - |
dc.author.google | Lee, JW | - |
dc.author.google | Park, JW | - |
dc.author.google | Choi, KY | - |
dc.relation.volume | 137 | - |
dc.relation.issue | 10 | - |
dc.relation.startpage | 2421 | - |
dc.relation.lastpage | 2430 | - |
dc.contributor.id | 10053210 | - |
dc.relation.journal | ANALYST | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Analyst, v.137, no.10, pp.2421 - 2430 | - |
dc.identifier.wosid | 000303126000024 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 2430 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 2421 | - |
dc.citation.title | Analyst | - |
dc.citation.volume | 137 | - |
dc.contributor.affiliatedAuthor | Park, JW | - |
dc.contributor.affiliatedAuthor | Choi, KY | - |
dc.identifier.scopusid | 2-s2.0-84864185980 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 11 | - |
dc.description.scptc | 10 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject.keywordPlus | ORIENTED IMMOBILIZATION | - |
dc.subject.keywordPlus | ANTIBODY FRAGMENTS | - |
dc.subject.keywordPlus | MICROARRAYS | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordPlus | CONSTRUCTION | - |
dc.subject.keywordPlus | DOMAINS | - |
dc.subject.keywordPlus | ARRAYS | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
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